Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification
To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill eff...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf |
| _version_ | 1796875293004136448 |
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| author | Rashid, Shah S, Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh |
| author_facet | Rashid, Shah S, Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh |
| author_sort | Rashid, Shah S, |
| collection | IIUM |
| description | To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain Trichoderma reesei RUT C-30. The study was conducted by using two different types of agents such as physical and chemical. Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for pretreatment of EFB to enhance the enzymatic saccharification of EFB. Among the physical pretreatment agents drying of EFB was found to be the best method and the maximum reducing sugar 99.06 mg/gm of EFB after 3 days (72 hours) was hydrolyzed from EFB which is 1.3 fold higher than the control. NaOH was proved to be the best among all the agents used in the pretreatment of EFB and 3% NaOH was far more effective on the enzymatic saccharification of EFB and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/gm of EFB) after 96 hours of incubation. |
| first_indexed | 2024-03-05T22:36:40Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:5686 |
| institution | International Islamic University Malaysia |
| language | English English |
| last_indexed | 2024-03-05T22:36:40Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:56862021-06-29T02:16:38Z http://irep.iium.edu.my/5686/ Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification Rashid, Shah S, Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh TP Chemical technology TP248.13 Biotechnology To achieve an accomplished optimized condition for enzymatic saccharification of palm oil mill empty fruit bunches (EFB) for higher yield of sugar hydrolysis, a comprehensive pretreatment of EFB was carried out using the laboratory produced cellulase enzyme through bioconversion of palm oil mill effluent (POME) by the fungal strain Trichoderma reesei RUT C-30. The study was conducted by using two different types of agents such as physical and chemical. Heating, boiling and steaming are among the physical agents and different concentrations of nitric acid, sulfuric acid and sodium hydroxide (NaOH) were the chemical agents used for pretreatment of EFB to enhance the enzymatic saccharification of EFB. Among the physical pretreatment agents drying of EFB was found to be the best method and the maximum reducing sugar 99.06 mg/gm of EFB after 3 days (72 hours) was hydrolyzed from EFB which is 1.3 fold higher than the control. NaOH was proved to be the best among all the agents used in the pretreatment of EFB and 3% NaOH was far more effective on the enzymatic saccharification of EFB and 2.35 fold increment was achieved on the yield of reducing sugar (175.03 mg/gm of EFB) after 96 hours of incubation. 2011-05-17 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf application/pdf en http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf Rashid, Shah S, and Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Hamzah, Mohd. Salleh (2011) Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Rashid, Shah S, Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamzah, Mohd. Salleh Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title | Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title_full | Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title_fullStr | Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title_full_unstemmed | Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title_short | Pretreatment of Empty Fruit Bunches (EFB) for improved Enzymatic Saccharification |
| title_sort | pretreatment of empty fruit bunches efb for improved enzymatic saccharification |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5686/1/ICBioE_2011_Rashid_et_al.pdf http://irep.iium.edu.my/5686/4/pretreatment_of_empty_fruit_bunches_%28EFB%29_for_improved_enxymatic_saccharification.pdf |
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