Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Medical Associations
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf |
| _version_ | 1796875324379627520 |
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| author | Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah |
| author_facet | Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah |
| author_sort | Mel, Maizirwan |
| collection | IIUM |
| description | The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed,
microcarrier concentration, and pO2 level are applied. |
| first_indexed | 2024-03-05T22:37:06Z |
| format | Article |
| id | oai:generic.eprints.org:5943 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:37:06Z |
| publishDate | 2010 |
| publisher | Malaysian Medical Associations |
| record_format | dspace |
| spelling | oai:generic.eprints.org:59432011-11-29T07:31:35Z http://irep.iium.edu.my/5943/ Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah TP248.13 Biotechnology The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO2 level turned out as the most significant parameter in the experiment. As predicted by analysis, cells grew up to 1.190 x 106 cells/ml when moderate agitation speed, microcarrier concentration, and pO2 level are applied. Malaysian Medical Associations 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Sohif, Hajar Naemah and Syed Hasan, Sharifah (2010) Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor. Medical Journal of Malaysia, 65 . pp. 19-21. ISSN 0300-5283 |
| spellingShingle | TP248.13 Biotechnology Mel, Maizirwan Arifin, Mohd Azmir Sohif, Hajar Naemah Syed Hasan, Sharifah Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title | Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title_full | Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title_fullStr | Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title_full_unstemmed | Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title_short | Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor |
| title_sort | optimization of process conditions for high cell density proliferation of df 1 cells in bioreactor |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf |
| work_keys_str_mv | AT melmaizirwan optimizationofprocessconditionsforhighcelldensityproliferationofdf1cellsinbioreactor AT arifinmohdazmir optimizationofprocessconditionsforhighcelldensityproliferationofdf1cellsinbioreactor AT sohifhajarnaemah optimizationofprocessconditionsforhighcelldensityproliferationofdf1cellsinbioreactor AT syedhasansharifah optimizationofprocessconditionsforhighcelldensityproliferationofdf1cellsinbioreactor |