Optimization of serum preparation in Vero cell culture using a statistical approach
Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA softwa...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Universiti Malaysia Sabah
2009
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf |
| _version_ | 1796875363160162304 |
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| author | Ahmad Nor, Yusilawati Nuhu Jaafar, Jaafar Mel, Maizirwan |
| author_facet | Ahmad Nor, Yusilawati Nuhu Jaafar, Jaafar Mel, Maizirwan |
| author_sort | Ahmad Nor, Yusilawati |
| collection | IIUM |
| description | Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500ml whole blood from disease free cattle using 50ml anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30min at 56oC. Vero cells were cultured in 25cm2 T flasks with an initial cell seeding of 4.2 x 105 cells/ml. Results obtained showed that locally made serum is
comparable in terms of maximum viable cell numbers (8.0 x 105 cells/ml) compared to commercial bovine serum (8.5x 105 cell/ml and 6.78x105 cell/ml) for GIBCO and PAA respectively after four days. From the study, locally
prepared serum can be substituted to the commercial sera for Vero cell culture. |
| first_indexed | 2024-03-05T22:37:40Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:6300 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:37:40Z |
| publishDate | 2009 |
| publisher | Universiti Malaysia Sabah |
| record_format | dspace |
| spelling | oai:generic.eprints.org:63002019-01-14T01:43:34Z http://irep.iium.edu.my/6300/ Optimization of serum preparation in Vero cell culture using a statistical approach Ahmad Nor, Yusilawati Nuhu Jaafar, Jaafar Mel, Maizirwan TP155 Chemical engineering TP248.13 Biotechnology Serum is a vital supplement in animal cell culture. Serum used in animal cell culture is from bovine source marketed by different companies, and somewhat expensive. The aims of this work were serum treatment and screening for the best serum concentration for Vero cell culture using STATISTICA software. The best result obtained from the software was used to compare with different commercial serum (GIBCOTM and PAA). Local serum was prepared first by aseptically collecting 500ml whole blood from disease free cattle using 50ml anticoagulant free centrifuge tubes. Whole blood was centrifuged to separate serum and blood cells. Serum collected was treated with ultraviolet radiation and heat inactivated for 30min at 56oC. Vero cells were cultured in 25cm2 T flasks with an initial cell seeding of 4.2 x 105 cells/ml. Results obtained showed that locally made serum is comparable in terms of maximum viable cell numbers (8.0 x 105 cells/ml) compared to commercial bovine serum (8.5x 105 cell/ml and 6.78x105 cell/ml) for GIBCO and PAA respectively after four days. From the study, locally prepared serum can be substituted to the commercial sera for Vero cell culture. Universiti Malaysia Sabah 2009-08-14 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf Ahmad Nor, Yusilawati and Nuhu Jaafar, Jaafar and Mel, Maizirwan (2009) Optimization of serum preparation in Vero cell culture using a statistical approach. In: 3rd International Conference on Chemical & Bioprocess Engineering , 12th–14th August 2009, Kota Kinabalu, Sabah. (Unpublished) |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Ahmad Nor, Yusilawati Nuhu Jaafar, Jaafar Mel, Maizirwan Optimization of serum preparation in Vero cell culture using a statistical approach |
| title | Optimization of serum preparation in Vero cell culture using a statistical approach |
| title_full | Optimization of serum preparation in Vero cell culture using a statistical approach |
| title_fullStr | Optimization of serum preparation in Vero cell culture using a statistical approach |
| title_full_unstemmed | Optimization of serum preparation in Vero cell culture using a statistical approach |
| title_short | Optimization of serum preparation in Vero cell culture using a statistical approach |
| title_sort | optimization of serum preparation in vero cell culture using a statistical approach |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/6300/1/Opt_serum_Preparation.pdf |
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