Fabrication of ceramic membrane chromatography for biologics purification
Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study,...
| Главные авторы: | , , |
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| Формат: | Proceeding Paper |
| Язык: | English |
| Опубликовано: |
2009
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| Предметы: | |
| Online-ссылка: | http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf |
| _version_ | 1825644767484051456 |
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| author | Mel, Maizirwan Abdeen, Fouad Sopyan, Iis |
| author_facet | Mel, Maizirwan Abdeen, Fouad Sopyan, Iis |
| author_sort | Mel, Maizirwan |
| collection | IIUM |
| description | Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature. |
| first_indexed | 2024-03-05T22:37:40Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:6301 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:37:40Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:63012011-11-21T19:52:37Z http://irep.iium.edu.my/6301/ Fabrication of ceramic membrane chromatography for biologics purification Mel, Maizirwan Abdeen, Fouad Sopyan, Iis TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology Chromatography is one of the most important separation processes of choice for the recovery/purification of proteins and complex bio-structures. Fabrication of chromatographic membranes and their efficiency in the chromatography process has been the subject of many recent researches. In this study, a coin-like, 13 mm diameter and 3 mm thick, ceramic membrane was fabricated to be used as a chromatographic medium. The membrane is used to replace the conventional resin-based chromatography columns. Hydroxyapatite (HA) powder was used as a material for the membrane fabrication. In this project, a HA powder was produced using starch as pore creating agents. Characterization processes were done for the ceramic membrane using the suitable apparatuses. Three parameters of the fabrication process (starch wt %, compaction pressure and sintering temperature) were manipulated to optimize the performance of the membrane. The fabricated membrane was placed in a (FPLC) system to be tested for its performance as an adsorptive membrane. (IMAC) process was run by immobilizing Ni2+ ions at the membrane particles surfaces. NP protein of the (NDV) was used to test the membrane’s ability to bind Histidine-tagged proteins. The optimum set of process parameters that yielded in the highest porosity and good chromatogram was determined to be 5 wt % starch, 3000 psi compaction pressure and 1100°C sintering temperature. 2009-10-29 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf Mel, Maizirwan and Abdeen, Fouad and Sopyan, Iis (2009) Fabrication of ceramic membrane chromatography for biologics purification. In: Advanced materials and Processing Technologies, 26-29 October 2009, Kuala Lumpur. |
| spellingShingle | TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology Mel, Maizirwan Abdeen, Fouad Sopyan, Iis Fabrication of ceramic membrane chromatography for biologics purification |
| title | Fabrication of ceramic membrane chromatography for biologics purification |
| title_full | Fabrication of ceramic membrane chromatography for biologics purification |
| title_fullStr | Fabrication of ceramic membrane chromatography for biologics purification |
| title_full_unstemmed | Fabrication of ceramic membrane chromatography for biologics purification |
| title_short | Fabrication of ceramic membrane chromatography for biologics purification |
| title_sort | fabrication of ceramic membrane chromatography for biologics purification |
| topic | TP155 Chemical engineering TP200 Manufacture and use of chemicals TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/6301/1/Paper_ID891_AMPT_2009.pdf |
| work_keys_str_mv | AT melmaizirwan fabricationofceramicmembranechromatographyforbiologicspurification AT abdeenfouad fabricationofceramicmembranechromatographyforbiologicspurification AT sopyaniis fabricationofceramicmembranechromatographyforbiologicspurification |