Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain
It is important to know the thermal and storage behavior of produced protein for the purpose of storage, transport and shelve life during industrial application. Differential Scanning Calorimetry (DSC) is used to measure the thermal and storage stability of recombinant bromelain produced i...
| Main Authors: | , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7470/1/10.BPE1_pg414-460_7470.pdf |
| _version_ | 1825644911509110784 |
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| author | Ismail, Nurul Azira Amid, Azura |
| author_facet | Ismail, Nurul Azira Amid, Azura |
| author_sort | Ismail, Nurul Azira |
| collection | IIUM |
| description | It is important to know the thermal and storage
behavior of produced protein for the purpose of storage,
transport and shelve life during industrial application.
Differential Scanning Calorimetry (DSC) is used to
measure the thermal and storage stability of recombinant
bromelain produced in our laboratory. Recombinant
bromelain thermal and storage stability were measured
and compared to the commercial bromelain. The purified
recombinant bromelain was powderized using freeze
drying method and its transition midpoint, crystalline and
enthalpy were recorded. Recombinant bromelain is more
stable than commercial bromelain at higher temperature
but the stability reduced after 7 days of storage at 4oC.
Moreover, its activity is lower than commercial at day 1
and 7. Higher energy is needed to break the bond between
amino acid chains in recombinant bromelain showing by
the enthalpy recorded suggesting that recombinant
bromelain has good protein structure and conformation
compared to commercial. |
| first_indexed | 2024-03-05T22:39:50Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:7470 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:39:50Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:74702012-01-19T03:40:53Z http://irep.iium.edu.my/7470/ Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain Ismail, Nurul Azira Amid, Azura Q Science (General) It is important to know the thermal and storage behavior of produced protein for the purpose of storage, transport and shelve life during industrial application. Differential Scanning Calorimetry (DSC) is used to measure the thermal and storage stability of recombinant bromelain produced in our laboratory. Recombinant bromelain thermal and storage stability were measured and compared to the commercial bromelain. The purified recombinant bromelain was powderized using freeze drying method and its transition midpoint, crystalline and enthalpy were recorded. Recombinant bromelain is more stable than commercial bromelain at higher temperature but the stability reduced after 7 days of storage at 4oC. Moreover, its activity is lower than commercial at day 1 and 7. Higher energy is needed to break the bond between amino acid chains in recombinant bromelain showing by the enthalpy recorded suggesting that recombinant bromelain has good protein structure and conformation compared to commercial. 2011-05-17 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/7470/1/10.BPE1_pg414-460_7470.pdf Ismail, Nurul Azira and Amid, Azura (2011) Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | Q Science (General) Ismail, Nurul Azira Amid, Azura Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title | Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title_full | Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title_fullStr | Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title_full_unstemmed | Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title_short | Differential Scanning Calorimetry as tool in observing thermal and storage stability of recombinant Bromelain |
| title_sort | differential scanning calorimetry as tool in observing thermal and storage stability of recombinant bromelain |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/7470/1/10.BPE1_pg414-460_7470.pdf |
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