| Summary: | This research was conducted to investigate the effect of chorionic gonadotrophin addition into maturation medium on oocyte maturation, fertilization, and embryo development in vitro on the Ettawa crossbred. Oocytes were divided 3 groups, group I: maturation medium without added chorionic gonadotrophin (0), group II: 10 µl/10 ml chorionic gonadotrophin was added into maturation medium (1), group III: 20 µl/10 ml chorionic gonadotrophin was added into maturation medium (2). Oocytes were transferred into 50 µl maturation medium, then covered by mineral oil. Oocyte was incubated on 39oC, 5% CO2, 95% humidity for 24 hours for maturation. Matured oocytes were inseminated with frozen semen – thawed concentration 12.5 x 106 /ml. Process of fertilization were carried out on incubator 39oC, 5% CO2, 95% humidity for 5 hours. The fertilized oocytes were transferred into 50 µl drop G – 1, then incubated on 39oC, 5% CO2, 95% humidity. Embryo development was monitored every 24 hours. Culture medium was changed every 48 hours. G – 2 medium used second day after culture. The variable measured involved oocyte maturation, fertilization, and in vitro cleavage rate. The data were analyzed by chi – square, using SPSS 15.0 program. Result showed no significant difference on the percentage of mature oocytes and fertilization rate were 78.0%, 72.8%, 75.0% and 76.6%, 74.5%, 77.8% respectively. But cleavage rate showed significant difference (P≤0.05) were 40.8%, 11.4%, 12.2% respectively. Based on the result it could be concluded that chorionic gonadotrophin addition into maturation medium had not increase ettawa crossbred oocytes on maturation, fertilization, and in vitro cleavage rate. The best maturation, fertilization, and in vitro cleavage rate is used maturation medium without added chorionic gonadotrophin.
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