A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana
The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA i...
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| Format: | Article |
| Language: | English |
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Asian Network for Scientific Information
2009
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| Online Access: | http://irep.iium.edu.my/864/1/144-152.pdf |
| _version_ | 1796874712850104320 |
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| author | Amid, Azura Warren, Garreth J. |
| author_facet | Amid, Azura Warren, Garreth J. |
| author_sort | Amid, Azura |
| collection | IIUM |
| description | The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis.
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| first_indexed | 2024-03-05T22:28:22Z |
| format | Article |
| id | oai:generic.eprints.org:864 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:28:22Z |
| publishDate | 2009 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | oai:generic.eprints.org:8642011-08-05T07:21:29Z http://irep.iium.edu.my/864/ A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana Amid, Azura Warren, Garreth J. TP248.13 Biotechnology The sfr3-1 mutation causes freezing-sensitivity in Arabidopsis thaliana. Through mapping, sequencing and transgenic complementation, sfr3-1 was revealed as a missense mutation in ACC1, which is an essential gene encoding multifunctional acetyl-CoA carboxylase. Suppression of ACC1 expression by RNA interference produced a freezing-sensitive phenotype with some similarity to that of sfr3-1. The dCAPS primers and PCR confirmed that sfr3 gene encodes multifunctional acetyl-CoA carboxylase. Microarray and real-time PCR experiments demonstrated that the expression of ACC1 increase only 1.48 fold in wild-type and 1.35 fold in mutant in response to cold treatment. Studies also suggested that the sfr3-1 mutation is more likely to be a temperature-sensitive mutation as the sfr3 mutant cuticle becomes leaky only at low temperature and this was confirmed by cuticular defects analysis. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/864/1/144-152.pdf Amid, Azura and Warren, Garreth J. (2009) A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana. International Journal of Botany, 5 (2). pp. 144-152. ISSN 1811-9719 (O), 1811-9700 (P) http://scialert.net/abstract/?doi=ijb.2009.144.152 DOI: 10.3923/ijb.2009.144.152 |
| spellingShingle | TP248.13 Biotechnology Amid, Azura Warren, Garreth J. A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title | A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title_full | A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title_fullStr | A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title_full_unstemmed | A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title_short | A multifunctional acetyl-CoA carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| title_sort | multifunctional acetyl coa carboxylase gene confers freesing tolerance in arabidopsis thaliana |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/864/1/144-152.pdf |
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