PCR-based chromosome walking: the SFR 3 case
In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Asian Network for Scientific Information
2006
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf |
| _version_ | 1796874717405118464 |
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| author | Amid, Azura Warren, Garreth J. Bramley, Peter M. |
| author_facet | Amid, Azura Warren, Garreth J. Bramley, Peter M. |
| author_sort | Amid, Azura |
| collection | IIUM |
| description | In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in the fine mapping experiments. The fine mapping experiments left the SFR3 region amongst 26 genes. Finally, 13 pseudogenes and transposable elements were eliminated and this left the SFR3 gene region amongst 13 genes in a 0.19 Mb region, which is represented by 4 BAC clones. The PCR-based chromosome mapping experiments to fine map the SFR3 gene have successfully decreased the SFR3 region from 2.7 to 0.19 Mb. We concluded that PCR-based chromosome walking is a convenient method to clone SFR3 gene. |
| first_indexed | 2024-03-05T22:28:23Z |
| format | Article |
| id | oai:generic.eprints.org:868 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:28:23Z |
| publishDate | 2006 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | oai:generic.eprints.org:8682011-08-05T07:32:50Z http://irep.iium.edu.my/868/ PCR-based chromosome walking: the SFR 3 case Amid, Azura Warren, Garreth J. Bramley, Peter M. TP248.13 Biotechnology In this study, mapping experiments were initiated in a region of 34.23 cM or 2.7 Mb to map the SFR3 gene into the smallest possible region on chromosome I. Eleven new PCR markers had been designed to fine map the gene. Roughly 557 F2 plants tested in these experiments and 38 lines were then used in the fine mapping experiments. The fine mapping experiments left the SFR3 region amongst 26 genes. Finally, 13 pseudogenes and transposable elements were eliminated and this left the SFR3 gene region amongst 13 genes in a 0.19 Mb region, which is represented by 4 BAC clones. The PCR-based chromosome mapping experiments to fine map the SFR3 gene have successfully decreased the SFR3 region from 2.7 to 0.19 Mb. We concluded that PCR-based chromosome walking is a convenient method to clone SFR3 gene. Asian Network for Scientific Information 2006 Article PeerReviewed application/pdf en http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf Amid, Azura and Warren, Garreth J. and Bramley, Peter M. (2006) PCR-based chromosome walking: the SFR 3 case. Pakistan Journal of Biological Sciences, 9 (1). pp. 164-169. ISSN 1812-5735 (O), 1028-8880 (P) http://docsdrive.com/pdfs/ansinet/pjbs/2006/164-169.pdf |
| spellingShingle | TP248.13 Biotechnology Amid, Azura Warren, Garreth J. Bramley, Peter M. PCR-based chromosome walking: the SFR 3 case |
| title | PCR-based chromosome walking: the SFR 3 case |
| title_full | PCR-based chromosome walking: the SFR 3 case |
| title_fullStr | PCR-based chromosome walking: the SFR 3 case |
| title_full_unstemmed | PCR-based chromosome walking: the SFR 3 case |
| title_short | PCR-based chromosome walking: the SFR 3 case |
| title_sort | pcr based chromosome walking the sfr 3 case |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/868/1/PCR-based_chromosome_walking.pdf |
| work_keys_str_mv | AT amidazura pcrbasedchromosomewalkingthesfr3case AT warrengarrethj pcrbasedchromosomewalkingthesfr3case AT bramleypeterm pcrbasedchromosomewalkingthesfr3case |