| Summary: | Background. Combination of gypsum and carbonated hydroxyapatite has been
shown to improve the bone healing process since gypsum that has been applied in
many clinical practice may fill irregular and small defect and evoke minimal
inflammatory response while carbonated hydroxyapatite has excellent
osteoconductivity and good resorbtion rate. Soft tissue biocompatibility test is
required prior to clinical application of bone substitutes since the material also
contact to the soft tissue upon application and soft tissue response are more severe
than the bone tissue response.
Objective. The aim of this study was to evaluate histocompatibility of gypsum
compare to combination gypsum with carbonated hydroxyapatite in subcutaneous
tissue.
Materials and Methods. Disk of gypsum and gypsum with carbonated
hydroxyapatite were made with diameter of 6 mm and 0.8 mm thick. Twenty five
healthy 3.5 � 4 month male Wistar rats were used. Each rat received one disk of
gypsum and one disk of gypsum with carbonated hydroxyapatite in paravertebral
subcutaneous tissue. Five rats were sacrificed each after 6 h, 5, 7, 14, and 21 days.
10 um paraffin sections of the implant and the tissue surround it were stained with
Hematoxylin Eosin. Histological evaluation and scoring with a histological
grading scale for soft-tissue implants were performed. A two-way ANOVA were
used to analyze the results of the histomorphometrical analysis. The level of
significance was set at P<0.05.
Results and discussions. Acute inflammatory cells were found in 6 h, 5 and 7 d
groups at similar number in both material. Chronic inflammatory cells such as
foreign giant cells and lymphocytes as well as capsule were observed in day
7,14,21. There are no significant different (p>0.05) of histomorphometrics score
between gypsum and gypsum with carbonated hydroxyapatite in each
implantation periods (7, 14, and 21 days).
Conclutions. Gypsum and combination between gypsum with carbonated
hydroxyapatit e have the same soft tissue histocompatibility level
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