DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA
Genetic diversity is a major information for conservation actions. It reflects the potential evolution for adaptation species or populations to environmental changes, and nowadays becoming a central topic in conservation genetics. The aims of this research were to find out the genetic diversity of t...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
|
| Subjects: |
| _version_ | 1826043493477253120 |
|---|---|
| author | , I Made Budiarsa, Drs.,M.Si. , Prof. Dr. Jesmandt Situmorang, M.Sc. |
| author_facet | , I Made Budiarsa, Drs.,M.Si. , Prof. Dr. Jesmandt Situmorang, M.Sc. |
| author_sort | , I Made Budiarsa, Drs.,M.Si. |
| collection | UGM |
| description | Genetic diversity is a major information for conservation actions. It reflects the potential
evolution for adaptation species or populations to environmental changes, and nowadays
becoming a central topic in conservation genetics. The aims of this research were to find
out the genetic diversity of the maleo from its natural habitat, to study the phylogenetic
relationship between maleo from two natural habitats and alternate incubation strategies,
and to study the maleo taxonomic affiliation and its phylogenetic relationship within
group of Megapodes, based on DNA sequences of RDP1 and ND2. Whole genome DNA
was extracted from 15 blood samples of maleo obtained from different habitats, coastal
and forest, according to the protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it
was used as template for amplification of RDP1 and ND2 by using PCR. The DNA
amplification was conducted according to the protocol of Takara Ex TaqTM PCR Kit in a
Perkin-Elmer Thermal Cycler 9600. PCR products were then purified using a Qiaquick
PCR Purification Kit (Qiagen, USA), and were used as template for sequencing reaction
following the protocol of BigDye v.3.1. Cycle Sequencing Kit using an ABI Prism 3100-
Avant Genetic Analyzer (Applied Biosystems, USA). Multiple alignments of DNA
sequencing results and sequences obtained from the international DNA data bases were
conducted by using Clustal-X. Characters of RDP1 and ND2 were analyzed by DnaSP
Version 3.51. Phylogenetic trees were constructed by using NJ algorithm with Juke-
Cantor evolution model from Phylip and K2P evolution model from MEGA Version 4.
The nucleotides similarity and nucleotides differences were analyzed using Phydith. The
analysis showed that mitochondrial ND2 retained better characters, higher number of
polymorphic sites and nucleotide diversity, compared to nuclear RDP1, which could be
used to distinguish population maleo in coastal and forest. RDP1 and ND2 gene
sequences presented congruent resolving power which places maleo as a monophyly of
Genus Macrocephalon, not a separated species.
Phylogenetic analysis based on ND2 characters produced a well resolved phylogenetic
tree compared with three major clades that showed a grouping of maleo from in-land and
coastal habitat. Philogenetic analysisis based on both RDP1 and ND2 gene sequences
showed that maleo was closely related to Aepypodius, Talegalla, Leipoa, Alectura and
this result supported current taxonomic status of Macrocephalon as a separate genus from
the Megapodius. |
| first_indexed | 2024-03-13T22:04:35Z |
| format | Thesis |
| id | oai:generic.eprints.org:88618 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:04:35Z |
| publishDate | 2011 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:886182014-08-20T02:51:52Z https://repository.ugm.ac.id/88618/ DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA , I Made Budiarsa, Drs.,M.Si. , Prof. Dr. Jesmandt Situmorang, M.Sc. ETD Genetic diversity is a major information for conservation actions. It reflects the potential evolution for adaptation species or populations to environmental changes, and nowadays becoming a central topic in conservation genetics. The aims of this research were to find out the genetic diversity of the maleo from its natural habitat, to study the phylogenetic relationship between maleo from two natural habitats and alternate incubation strategies, and to study the maleo taxonomic affiliation and its phylogenetic relationship within group of Megapodes, based on DNA sequences of RDP1 and ND2. Whole genome DNA was extracted from 15 blood samples of maleo obtained from different habitats, coastal and forest, according to the protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplification of RDP1 and ND2 by using PCR. The DNA amplification was conducted according to the protocol of Takara Ex TaqTM PCR Kit in a Perkin-Elmer Thermal Cycler 9600. PCR products were then purified using a Qiaquick PCR Purification Kit (Qiagen, USA), and were used as template for sequencing reaction following the protocol of BigDye v.3.1. Cycle Sequencing Kit using an ABI Prism 3100- Avant Genetic Analyzer (Applied Biosystems, USA). Multiple alignments of DNA sequencing results and sequences obtained from the international DNA data bases were conducted by using Clustal-X. Characters of RDP1 and ND2 were analyzed by DnaSP Version 3.51. Phylogenetic trees were constructed by using NJ algorithm with Juke- Cantor evolution model from Phylip and K2P evolution model from MEGA Version 4. The nucleotides similarity and nucleotides differences were analyzed using Phydith. The analysis showed that mitochondrial ND2 retained better characters, higher number of polymorphic sites and nucleotide diversity, compared to nuclear RDP1, which could be used to distinguish population maleo in coastal and forest. RDP1 and ND2 gene sequences presented congruent resolving power which places maleo as a monophyly of Genus Macrocephalon, not a separated species. Phylogenetic analysis based on ND2 characters produced a well resolved phylogenetic tree compared with three major clades that showed a grouping of maleo from in-land and coastal habitat. Philogenetic analysisis based on both RDP1 and ND2 gene sequences showed that maleo was closely related to Aepypodius, Talegalla, Leipoa, Alectura and this result supported current taxonomic status of Macrocephalon as a separate genus from the Megapodius. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , I Made Budiarsa, Drs.,M.Si. and , Prof. Dr. Jesmandt Situmorang, M.Sc. (2011) DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50885 |
| spellingShingle | ETD , I Made Budiarsa, Drs.,M.Si. , Prof. Dr. Jesmandt Situmorang, M.Sc. DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title | DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title_full | DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title_fullStr | DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title_full_unstemmed | DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title_short | DIVERSITAS GENETIK DAN FILOGENI BURUNG MALEO (Mavrocephalon maleo S. Muller) BERDASARKAN SEKUEN GEN RDPI NUKLEUS DAN GEN ND2 MITOKONDRIA |
| title_sort | diversitas genetik dan filogeni burung maleo mavrocephalon maleo s muller berdasarkan sekuen gen rdpi nukleus dan gen nd2 mitokondria |
| topic | ETD |
| work_keys_str_mv | AT imadebudiarsadrsmsi diversitasgenetikdanfilogeniburungmaleomavrocephalonmaleosmullerberdasarkansekuengenrdpinukleusdangennd2mitokondria AT profdrjesmandtsitumorangmsc diversitasgenetikdanfilogeniburungmaleomavrocephalonmaleosmullerberdasarkansekuengenrdpinukleusdangennd2mitokondria |