VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY DAN APLIKASINYA DALAM SEDIAAN KAPSUL
Gabapentine, is a new antiepileptic drug which has a structural analogue of neurotransmitter γ-aminobutyric acid (GABA). Gabapentine inhibits calcium uptake of gated calcium channels presinaps in the brain. This research aimed to develop and validate a new HPLC method with UV detection for determin...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2011
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| author | , nurmaya effendi , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_facet | , nurmaya effendi , Prof. Dr. Sudibyo Martono, M.S., Apt. |
| author_sort | , nurmaya effendi |
| collection | UGM |
| description | Gabapentine, is a new antiepileptic drug which has a structural analogue of
neurotransmitter γ-aminobutyric acid (GABA). Gabapentine inhibits calcium uptake
of gated calcium channels presinaps in the brain. This research aimed to develop and
validate a new HPLC method with UV detection for determination of gabapentine in
capsule.
The method is based on the reaction of gabapentine with 1-fluoro-2,4-
dinitrobenzen (FDNB) in acetonitrile which carried out in the presence of borate
buffer (pH = 9,5) at 65oC for 20 minutes, which formed a yellow colored product
with the absorption at 367 nm. The product was separated by HPLC using A Zorbax
ODS (Agilent) column (250 x 4.6 mm, 5 μm) was utilized as stationary phase and 50
mM acetate buffer (pH 3.7) : methanol : acetonitril (25 : 30 : 45 v/v/v) as mobile
phase, with flow rate 0.8 mL/min, and the detection at 367 nm.
The purposed method was validated by means of accuracy, precision,
linearity, selectivity, LOD and LOQ, and the complied with the requirement for
validity. The method has been succesfully applied for the determination of
gabapentine in capsule with LOD = 1.05 μg/mL and LOQ = 3.49, linear quantitative
response curve was generated over a concentration range of 3.49 - 100 μg/mL with
the correlation coefficient of 0.99996 and recovery range of 97.27 � 104.24% with
RSD 2.63%. |
| first_indexed | 2024-03-13T22:05:17Z |
| format | Thesis |
| id | oai:generic.eprints.org:88839 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:05:17Z |
| publishDate | 2011 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:888392014-08-20T02:54:38Z https://repository.ugm.ac.id/88839/ VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY DAN APLIKASINYA DALAM SEDIAAN KAPSUL , nurmaya effendi , Prof. Dr. Sudibyo Martono, M.S., Apt. ETD Gabapentine, is a new antiepileptic drug which has a structural analogue of neurotransmitter γ-aminobutyric acid (GABA). Gabapentine inhibits calcium uptake of gated calcium channels presinaps in the brain. This research aimed to develop and validate a new HPLC method with UV detection for determination of gabapentine in capsule. The method is based on the reaction of gabapentine with 1-fluoro-2,4- dinitrobenzen (FDNB) in acetonitrile which carried out in the presence of borate buffer (pH = 9,5) at 65oC for 20 minutes, which formed a yellow colored product with the absorption at 367 nm. The product was separated by HPLC using A Zorbax ODS (Agilent) column (250 x 4.6 mm, 5 μm) was utilized as stationary phase and 50 mM acetate buffer (pH 3.7) : methanol : acetonitril (25 : 30 : 45 v/v/v) as mobile phase, with flow rate 0.8 mL/min, and the detection at 367 nm. The purposed method was validated by means of accuracy, precision, linearity, selectivity, LOD and LOQ, and the complied with the requirement for validity. The method has been succesfully applied for the determination of gabapentine in capsule with LOD = 1.05 μg/mL and LOQ = 3.49, linear quantitative response curve was generated over a concentration range of 3.49 - 100 μg/mL with the correlation coefficient of 0.99996 and recovery range of 97.27 � 104.24% with RSD 2.63%. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , nurmaya effendi and , Prof. Dr. Sudibyo Martono, M.S., Apt. (2011) VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY DAN APLIKASINYA DALAM SEDIAAN KAPSUL. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50813 |
| spellingShingle | ETD , nurmaya effendi , Prof. Dr. Sudibyo Martono, M.S., Apt. VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title | VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI
SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title_full | VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI
SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title_fullStr | VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI
SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title_full_unstemmed | VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI
SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title_short | VALIDASI METODE PENETAPAN KADAR GABAPENTIN TERDERIVATISASI
SECARA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
DAN APLIKASINYA DALAM SEDIAAN KAPSUL |
| title_sort | validasi metode penetapan kadar gabapentin terderivatisasi secara high performance liquid chromatography dan aplikasinya dalam sediaan kapsul |
| topic | ETD |
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