| Summary: | The study of amylolytic bacteria which produce poly-β-hydroxybutyrate (PHB) from
sago starch was conducted to obtain the best indigenous amylolytic bacteria to be developed
as PHB producer from sago starch at industrial scale. The amylolytic bacteria were
selectively isolated using starch agar media and screened based on amylolytic activity. As
many as 117 isolates amylolytic bacteria were isolated from processing sago starch area in
Konawe regency, Southeast Sulawesi and 81 isolates among them were selected based on
the amylolytic activity to be further tested for PHB production capacity. Screening of the
amylolytic bacteria based on PHB producing capacity was done qualitatively by Sudan black
staining and quantitatively by the spectrophotometric method. Results of the screening
showed that 50 isolates among the 81 isolate bacteria were able to produce PHB ranged
from 0,22-52,28 % (g PHB/g cell dry weight). Three isolate bacteria were selected based on
amylolytic activity and PHB production capacity, namely isolate PPK5 had the highest
amylolytic activity and PSA10 together with PPK6 were the most potential PHB producer.
Amylase specific activity of the isolate PPK5, PSA10 and PPK6 was 5226,32
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