DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI MONOKLONAL DSSE10
Background: Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main target of surveillance and control activities. Virologic surveillan...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2011
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| _version_ | 1797030427715698688 |
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| author | , DYAH WIDIASTUTI, S.SI. , Dr. drh. Sitti Rahmah Umniyati, SU. |
| author_facet | , DYAH WIDIASTUTI, S.SI. , Dr. drh. Sitti Rahmah Umniyati, SU. |
| author_sort | , DYAH WIDIASTUTI, S.SI. |
| collection | UGM |
| description | Background: Dengue viruses, globally the most prevalent arboviruses, are
transmitted to humans by persistently infected Aedes mosquitoes. The most
important vector of Dengue virus is the mosquito Ae.aegypti, which should be the
main target of surveillance and control activities. Virologic surveillance for
dengue viruses in its vector has been used as an early warning system to predict
outbreaks. Detection of Dengue virus antigen in mosquito head squash using
immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an
alternative method for dengue vector surveillance. The study aimed to measure
sensitivity, specificity and interobserver agreement (kappa value) of
immunocytochemical SBPC assay to detect Dengue virus infection in head squash
of Ae.aegypti.
Methods: The study design was experimental. Artificially-infected adult
Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and noninfected
adult Ae. aegypti mosquitoes were used as normal ones. The
immunocytochemical SBPC assay using monoclonal antibody DSSE10 then was
applied in mosquito head squash to detect Dengue virus antigen. RT-PCR as a
gold standard was applied in mosquito thorax. The results were analyzed by
descriptive statistic test and 2x2 diagnostic test table.
Results: The immunocytochemical SBPC assay can detect Dengue virus antigen
in mosquito head squash at day 2 postinfection, while RT-PCR can detect at day 5
postifection. There are some false positive results found in immunocytochemical
SBPC assay. The sensitivity of immunocytochemical SBPC assay in mosquito
head squash was 100% with a specificity of 91%. Whilst the kappa value showed
a good agreement between two observers (kappa value 0,63).
Conclusions: The immunocytochemical SBPC assay in mosquito head squash has
high sensitivity, high specificity and good interobserver agreement. |
| first_indexed | 2024-03-13T22:05:38Z |
| format | Thesis |
| id | oai:generic.eprints.org:88923 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:05:38Z |
| publishDate | 2011 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:889232014-08-20T02:50:55Z https://repository.ugm.ac.id/88923/ DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI MONOKLONAL DSSE10 , DYAH WIDIASTUTI, S.SI. , Dr. drh. Sitti Rahmah Umniyati, SU. ETD Background: Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main target of surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an early warning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash using immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for dengue vector surveillance. The study aimed to measure sensitivity, specificity and interobserver agreement (kappa value) of immunocytochemical SBPC assay to detect Dengue virus infection in head squash of Ae.aegypti. Methods: The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and noninfected adult Ae. aegypti mosquitoes were used as normal ones. The immunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash to detect Dengue virus antigen. RT-PCR as a gold standard was applied in mosquito thorax. The results were analyzed by descriptive statistic test and 2x2 diagnostic test table. Results: The immunocytochemical SBPC assay can detect Dengue virus antigen in mosquito head squash at day 2 postinfection, while RT-PCR can detect at day 5 postifection. There are some false positive results found in immunocytochemical SBPC assay. The sensitivity of immunocytochemical SBPC assay in mosquito head squash was 100% with a specificity of 91%. Whilst the kappa value showed a good agreement between two observers (kappa value 0,63). Conclusions: The immunocytochemical SBPC assay in mosquito head squash has high sensitivity, high specificity and good interobserver agreement. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , DYAH WIDIASTUTI, S.SI. and , Dr. drh. Sitti Rahmah Umniyati, SU. (2011) DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI MONOKLONAL DSSE10. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=50724 |
| spellingShingle | ETD , DYAH WIDIASTUTI, S.SI. , Dr. drh. Sitti Rahmah Umniyati, SU. DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI MONOKLONAL DSSE10 |
| title | DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti
DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN
ANTIBODI MONOKLONAL DSSE10 |
| title_full | DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti
DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN
ANTIBODI MONOKLONAL DSSE10 |
| title_fullStr | DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti
DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN
ANTIBODI MONOKLONAL DSSE10 |
| title_full_unstemmed | DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti
DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN
ANTIBODI MONOKLONAL DSSE10 |
| title_short | DETEKSI INFEKSI VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti
DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN
ANTIBODI MONOKLONAL DSSE10 |
| title_sort | deteksi infeksi virus dengue 3 pada nyamuk aedes aegypti dengan teknik imunositokimia menggunakan antibodi monoklonal dsse10 |
| topic | ETD |
| work_keys_str_mv | AT dyahwidiastutissi deteksiinfeksivirusdengue3padanyamukaedesaegyptidenganteknikimunositokimiamenggunakanantibodimonoklonaldsse10 AT drdrhsittirahmahumniyatisu deteksiinfeksivirusdengue3padanyamukaedesaegyptidenganteknikimunositokimiamenggunakanantibodimonoklonaldsse10 |