EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN PEMACUAN APOPTOSIS
Combination of chemotherapeutic agent and chemopreventive agent is being a new approach in cancer treatment. This is aimed at enhancing the effectivity and also reducing drug resistance and adverse side effect of the chemotherapeutic agent. Hesperidin, a citrus flavonoid has been reported to reduce...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2011
|
| Subjects: |
| _version_ | 1797030519373824000 |
|---|---|
| author | , INDRI KUSHARYANTI, S.Farm, APT , Prof. Dr. Edy Meiyanto, M.Si., Apt. |
| author_facet | , INDRI KUSHARYANTI, S.Farm, APT , Prof. Dr. Edy Meiyanto, M.Si., Apt. |
| author_sort | , INDRI KUSHARYANTI, S.Farm, APT |
| collection | UGM |
| description | Combination of chemotherapeutic agent and chemopreventive agent is
being a new approach in cancer treatment. This is aimed at enhancing the
effectivity and also reducing drug resistance and adverse side effect of the
chemotherapeutic agent. Hesperidin, a citrus flavonoid has been reported to
reduce the proliferation of many cancer cells. The objectives of this study were to
investigate cytotoxic activities, cell cycle modulation and apoptosis induction of
hesperidin and its combination with doxorubicin on Hela cell lines. MTT [3-(4,5-
dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] assay was used to
measure the growth inhibitory effect of hesperidin and its combination with
doxorubicin on Hela cells. Cell cycle profile was determined by flowcytometry
and the data obtained was analyzed by using ModFit LT 3.0 program. Apoptosis
assay was done using double staining method using ethidium-bromide and
acridine-orange.
Hesperidin inhibited cell growth with IC50 48 μM, while the IC50 of
doxorubicin was 1 μM. Combination of 5 μM doxorubicin and 6 μM hesperidin
showed strongest inhibitory effect toward Hela cells which save 3,28% of cell
viability. Hesperidin of 24 µM accumulated HeLa cells at G1 phase, while
doxorubicin of 5 μM accumulated HeLa cells at S phase. Combination of 24 μM
hesperidin with 5 μM doxorubicin gave G1 and S phase accumulation at 24 h
incubation. All of Hesperidin and Doxorubicin were capable of inducing
apoptosis. Their combination increased apoptosis of HeLa sel compared with
single treatment. In accordance of this apoptosis induction effect,
imunocytochemistry method was conduct to determine the level of Bcl-2 and Bax
expression. It is showed that hesperidin, doxorubicin and their combination
decreases the expression Bcl-2 and increases the expression of Bax. According to
this results, hesperidin has a potency to be developed as co-chemotherapeutic
agent for cervical cancer. |
| first_indexed | 2024-03-13T22:07:04Z |
| format | Thesis |
| id | oai:generic.eprints.org:89380 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:07:04Z |
| publishDate | 2011 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:893802014-08-20T02:51:56Z https://repository.ugm.ac.id/89380/ EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN PEMACUAN APOPTOSIS , INDRI KUSHARYANTI, S.Farm, APT , Prof. Dr. Edy Meiyanto, M.Si., Apt. ETD Combination of chemotherapeutic agent and chemopreventive agent is being a new approach in cancer treatment. This is aimed at enhancing the effectivity and also reducing drug resistance and adverse side effect of the chemotherapeutic agent. Hesperidin, a citrus flavonoid has been reported to reduce the proliferation of many cancer cells. The objectives of this study were to investigate cytotoxic activities, cell cycle modulation and apoptosis induction of hesperidin and its combination with doxorubicin on Hela cell lines. MTT [3-(4,5- dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] assay was used to measure the growth inhibitory effect of hesperidin and its combination with doxorubicin on Hela cells. Cell cycle profile was determined by flowcytometry and the data obtained was analyzed by using ModFit LT 3.0 program. Apoptosis assay was done using double staining method using ethidium-bromide and acridine-orange. Hesperidin inhibited cell growth with IC50 48 μM, while the IC50 of doxorubicin was 1 μM. Combination of 5 μM doxorubicin and 6 μM hesperidin showed strongest inhibitory effect toward Hela cells which save 3,28% of cell viability. Hesperidin of 24 µM accumulated HeLa cells at G1 phase, while doxorubicin of 5 μM accumulated HeLa cells at S phase. Combination of 24 μM hesperidin with 5 μM doxorubicin gave G1 and S phase accumulation at 24 h incubation. All of Hesperidin and Doxorubicin were capable of inducing apoptosis. Their combination increased apoptosis of HeLa sel compared with single treatment. In accordance of this apoptosis induction effect, imunocytochemistry method was conduct to determine the level of Bcl-2 and Bax expression. It is showed that hesperidin, doxorubicin and their combination decreases the expression Bcl-2 and increases the expression of Bax. According to this results, hesperidin has a potency to be developed as co-chemotherapeutic agent for cervical cancer. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , INDRI KUSHARYANTI, S.Farm, APT and , Prof. Dr. Edy Meiyanto, M.Si., Apt. (2011) EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN PEMACUAN APOPTOSIS. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=51881 |
| spellingShingle | ETD , INDRI KUSHARYANTI, S.Farm, APT , Prof. Dr. Edy Meiyanto, M.Si., Apt. EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN PEMACUAN APOPTOSIS |
| title | EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS
SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER
RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN
PEMACUAN APOPTOSIS |
| title_full | EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS
SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER
RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN
PEMACUAN APOPTOSIS |
| title_fullStr | EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS
SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER
RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN
PEMACUAN APOPTOSIS |
| title_full_unstemmed | EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS
SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER
RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN
PEMACUAN APOPTOSIS |
| title_short | EFEK HESPERIDIN DALAM PENINGKATAN AKTIVITAS
SITOTOKSIK DOXORUBICIN PADA SEL KANKER LEHER
RAHIM HeLa MELALUI MODULASI SIKLUS SEL DAN
PEMACUAN APOPTOSIS |
| title_sort | efek hesperidin dalam peningkatan aktivitas sitotoksik doxorubicin pada sel kanker leher rahim hela melalui modulasi siklus sel dan pemacuan apoptosis |
| topic | ETD |
| work_keys_str_mv | AT indrikusharyantisfarmapt efekhesperidindalampeningkatanaktivitassitotoksikdoxorubicinpadaselkankerleherrahimhelamelaluimodulasisiklusseldanpemacuanapoptosis AT profdredymeiyantomsiapt efekhesperidindalampeningkatanaktivitassitotoksikdoxorubicinpadaselkankerleherrahimhelamelaluimodulasisiklusseldanpemacuanapoptosis |