| Summary: | Malaria is a life-threatening disease caused by Plasmodium parasites. The
rapid spread of malaria-quinoline resistance enforce a finding of new antimalaria
drug. Artemisia annua L plant that had artemisinin as secondary metabolic, had
been used as antimalaria agent for long time ago. One source of bioactive
compound is endophytic fungus. This fungus can produce the same or similar to
its host plant.
This research was done by isolation endophytic fungus, fermentation,
extraction of fermentation medium and fungus miselium, analysis chromatogram
with KLT, analysis of the haem polymerization inhibitory activity, analysis with
HPLC and LC-MS, analysis of active compound group, and fungus identification.
From 6 kind of fungus isolated from A. annua L., 3 kind of those fungus
(fungus A, E, and F) was guessed could produce intracellular artemisinin. Fungus
E had the highest value of the haem polymerization inhibitory activity with IC50
0,499 mg/mL. However, the analysis result of HPLC and LC-MS showed that
fungus E did not contain artemisinin or its derivates, such dihydroartemisinin,
arthemether, artheether, or artesunate. The result analysis of active compound
group showed that fungus E miselium was consisted of terpenoid group
compound, and this fungus was included in genus Tritirachium sp.
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