| Summary: | Background: Immune system could be specifically manipulated by vaccination
or non specifically by the use of immunomodulator. Flavonoid have been reported
to have some biological effects, one of them is immunomodulating effect. Bidens
pilosa L. contains flavonoid which has not been clarified yet whether it has effect
on cellular immune response.
Objectives: To evaluate the effect of water extract of B. pilosa L. on cellular
immune response in mice infected with Listeria monocytogenes
Method: Seventy two female Balb/c mice were divided into six groups, consist of
12 mice. The first and second group received aquadest only (negative control).
The third, fourth, and fifth group received B. pilosa L. water extract with
different dose, i.e. 125 mg/kgBW/day, 250 mg/kgBW/day, dan 500
mg/kgBW/day, respectively. The sixth group received Panax ginseng extract 26
mg/kgBW/day as a positive control. The test material were given everyday for 14
days. On 15
th
day, all groups except first group were infected with L.
monocytogenes. On 0, 2, 4, and 7 after bacterial infection, 3 mice of each group
were killed and then peritoneal macrophage and spleen lymphocyte were cultured.
Phagocytosis activity was measured by the ability of mice peritoneal macrophages
to phagocyte latex particles in vitro and lymphocyte proliferation activity was
determined using MTT assay method.
Results: B. pilosa L. water extract could increase cellular immune response in
mice against L. monocytogenes infection, i.e. macrophage phagocytosis and
spleen lymphocyte proliferation. There were a significant different between
negative control group and group treated with 500 mg/kgBW/day of B. pilosa L.
water extract.
Conclusions: The water extract of Bidens pilosa L. enhanced the activity of
macrophage phagocytosis and lymphocyte proliferation.
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