| Summary: | Immunomodulator is an agent that helps to maintain immune system. Ants
plant belongs to rubiaceae family that is widely used by Indonesian people as part
of traditional medicine for various diseases. Wide therapeutic effects suggest its
immunomodulatory activity as part of healing properties. The purpose of this
study was to investigate the immunomodulatory activity and to identify the active
compound of the ants plant (Myrmecodia pendens Merr & Perry) by an in vitro
technique.
Dried powders of ant plant were extracted by using maceration method with
ethanol 95%. Extract obtained was then fractioned by liquid-liquid partition using
n-hexane, ethyl acetate and water, excessively. Extracts and fractions were tested
against lymphocyte proliferation and macrophage phagocytosis. Lymphocytes and
macrophages were isolated from Balb/c male mice 2 months old. The most active
fraction was further fractionated by using two different method, i.e: column
chromatography (sephadex LH20, metanol) and VLC (silica gel, increasing
polarity of solvent started from n-hexane followed by ethyl acetate and ended by
methanol). The fractions yielded were further tested by lymphocyte proliferation
assay using MTT assay. Each fractions was analysed the total phenolic contents
by using Folin-Ciocalteau method. Active compound was identified by GC-MS,
UV-Vis spectroscopy, IR and 1H-NMR.
The study result showed that phenolic compounds played a role in
lymphocyte proliferation induction. Fraction 7 yielded from VLC (ethyl acetatemethanol
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