ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMIKROBA DARI UMBI SARANG SEMUT (Myrmecodia tuberosa (non Jack.) BI)
Sarang-semut tubers (Myrmecodia tuberosa (non Jack.) BI) has been widely used as part of traditional medicine against various diseases in West Papua. Recently the extract has been reported to be active as antimicrobes. The purpose of this study was isolate antimicrobial compound and to determine the...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2011
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| _version_ | 1826043810670444544 |
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| author | , Wahyuning Setyani, S.Si., Apt. , Dr.rer.nat. Triana Hertiani, M.Si.,Apt. |
| author_facet | , Wahyuning Setyani, S.Si., Apt. , Dr.rer.nat. Triana Hertiani, M.Si.,Apt. |
| author_sort | , Wahyuning Setyani, S.Si., Apt. |
| collection | UGM |
| description | Sarang-semut tubers (Myrmecodia tuberosa (non Jack.) BI) has been
widely used as part of traditional medicine against various diseases in West
Papua. Recently the extract has been reported to be active as antimicrobes. The
purpose of this study was isolate antimicrobial compound and to determine the
characteristics of isolate to contained in Myrmecodia tuberosa (non Jack.) BI.
Extraction of dried powder was performed by maceration method using
95% ethanol. Ethanol extract obtained was concentrated and then fractionated
with n-hexane, ethyl acetate, and water successively. Concentrated extracts and
fractions were tested against Staphylococcus aureus, Escherichia coli, and
Candida albicans with Agar Diffusion technique.
Active fraction was further fractioned by TLC-Preparative based on
Bioautography data against sensitive microbe. After proven the purity by TLC
method, isolate was identified by using LCMS, and 1H-NMR. Results showed that
isolate was active against Staphylococcus aureus. It was identified to be a
phenolic compound with carboxylic and aliphatic alcohol at side chain. |
| first_indexed | 2024-03-13T22:09:39Z |
| format | Thesis |
| id | oai:generic.eprints.org:90192 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:09:39Z |
| publishDate | 2011 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:901922014-08-20T02:54:17Z https://repository.ugm.ac.id/90192/ ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMIKROBA DARI UMBI SARANG SEMUT (Myrmecodia tuberosa (non Jack.) BI) , Wahyuning Setyani, S.Si., Apt. , Dr.rer.nat. Triana Hertiani, M.Si.,Apt. ETD Sarang-semut tubers (Myrmecodia tuberosa (non Jack.) BI) has been widely used as part of traditional medicine against various diseases in West Papua. Recently the extract has been reported to be active as antimicrobes. The purpose of this study was isolate antimicrobial compound and to determine the characteristics of isolate to contained in Myrmecodia tuberosa (non Jack.) BI. Extraction of dried powder was performed by maceration method using 95% ethanol. Ethanol extract obtained was concentrated and then fractionated with n-hexane, ethyl acetate, and water successively. Concentrated extracts and fractions were tested against Staphylococcus aureus, Escherichia coli, and Candida albicans with Agar Diffusion technique. Active fraction was further fractioned by TLC-Preparative based on Bioautography data against sensitive microbe. After proven the purity by TLC method, isolate was identified by using LCMS, and 1H-NMR. Results showed that isolate was active against Staphylococcus aureus. It was identified to be a phenolic compound with carboxylic and aliphatic alcohol at side chain. [Yogyakarta] : Universitas Gadjah Mada 2011 Thesis NonPeerReviewed , Wahyuning Setyani, S.Si., Apt. and , Dr.rer.nat. Triana Hertiani, M.Si.,Apt. (2011) ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMIKROBA DARI UMBI SARANG SEMUT (Myrmecodia tuberosa (non Jack.) BI). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52907 |
| spellingShingle | ETD , Wahyuning Setyani, S.Si., Apt. , Dr.rer.nat. Triana Hertiani, M.Si.,Apt. ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMIKROBA DARI UMBI SARANG SEMUT (Myrmecodia tuberosa (non Jack.) BI) |
| title | ISOLASI DAN IDENTIFIKASI SENYAWA
ANTIMIKROBA DARI UMBI SARANG SEMUT
(Myrmecodia tuberosa (non Jack.) BI) |
| title_full | ISOLASI DAN IDENTIFIKASI SENYAWA
ANTIMIKROBA DARI UMBI SARANG SEMUT
(Myrmecodia tuberosa (non Jack.) BI) |
| title_fullStr | ISOLASI DAN IDENTIFIKASI SENYAWA
ANTIMIKROBA DARI UMBI SARANG SEMUT
(Myrmecodia tuberosa (non Jack.) BI) |
| title_full_unstemmed | ISOLASI DAN IDENTIFIKASI SENYAWA
ANTIMIKROBA DARI UMBI SARANG SEMUT
(Myrmecodia tuberosa (non Jack.) BI) |
| title_short | ISOLASI DAN IDENTIFIKASI SENYAWA
ANTIMIKROBA DARI UMBI SARANG SEMUT
(Myrmecodia tuberosa (non Jack.) BI) |
| title_sort | isolasi dan identifikasi senyawa antimikroba dari umbi sarang semut myrmecodia tuberosa non jack bi |
| topic | ETD |
| work_keys_str_mv | AT wahyuningsetyanissiapt isolasidanidentifikasisenyawaantimikrobadariumbisarangsemutmyrmecodiatuberosanonjackbi AT drrernattrianahertianimsiapt isolasidanidentifikasisenyawaantimikrobadariumbisarangsemutmyrmecodiatuberosanonjackbi |