Summary: | Various media compositions have been formulated to optimize germination
of orchid seed cultured and development of Spahtoglottis plicata plantlets.
Modifications of fertilizer solution such, Growmore and Hyponex with various
agar brand can be used on orchids seed culture. The objective of this research
were to germinate the orchid seed in cultured and development of S. plicata
plantlets by using the medium consist of fertilizer and agar brand, to determine
optimum concentration of fertilizer and agar brand in germination of orchid seed
cultured and development of S. plicata plantlets. The research was conducted
using Completely Randomized Design (CRD). The first factors are 5 media type
of inorganic fertilizer: Growmore 1 g/l (G1), Growmore 3 g/l (G3), Hyponex 1 g/l
(H1), Hyponex 3 g/l (H3) and Vacin & Went (VW) as control, and the second
factors are agar brands: Bioagar (B), Nutrijel (N) dan Swallow (S). Each
treatment consisted of 3 replications. Data were analyzed with Analyze of
Variance (Anova) a statistical test using Duncan Multiple Range Test (DMRT) at
5% level.
The results showed that the fertilizer and agar brand shown the highest of
germination percentage (97,64 and 96,17%) giving in medium H1S and VB:
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