| Summary: | Curcumin has various of biological activities with broad spectrum, but it
has low solubility in water resulting in low systemic bioavailability. This study
aims to formulate curcumin nanoparticles with medium-chain chitosan as a carrier
which is known to be stable, safe and able to enter the cells.
Preparation of curcumin-chitosan nanoparticles prepared by ionic gelation
method in an acetate buffer pH 4 and 5. Characterization was due to measure the
particle size and zeta potential, morphology of nanoparticle and to determined its
entrapment efficiency. The nanoparticle stability is determined by measuring the
free curcumin released in artificial intestinal fluid (AIF). The toxicity determined
by MTT method with Vero cells as the cell culture model. The cellular uptake on
T47D cells was observed using a fluorescent microscope.
The nanocurcumin prepared in acetate buffer pH 4 have an average
diameter of 563.77 nm with a zeta potential (+) 23 mV, the entrapment efficiency
is 70.25 to 90.02% and the stability in the AIF is 83,61 % to 85,36 %. MTT test
showed that nanocurcumin is not toxic to the Vero cell with viability > 80 % , and
observation by fluorescence microscopy showed that nanocurcumin is able to
enter the cell. Based on the results, it is concluded that nanoparticle formulations
of curcumin with medium-chain chitosan as a carrier is safe, stable and is able to
enter the cell.
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