| Summary: | The nanoparticle in transdermal drug delivery system has been widely
developed rapidly to increase skin permeability and to deliver drug into systemic
circulation. This research aim was to find out optimal condition of chitosan-losartan
nanoparticle and its effect on in vitro transdermal transport.
Ionic gelation method was used, and two variables were optimized, i.e. pH
(4,0-5,0) and rate of stirring (350-700 rpm). Loading capacity was used as a respon.
Experimental transdermal transport was carried out for 28 hours using vertical type
diffusion cell. Four formulas, i.e. Formula (F) I (losartan potassium solution in
water), F II (nanoparticle dispersion in water), F III (losartan potassium solution in
water with 10 % oleic acid in propilenglycol pretreatment), F IV (nanoparticle
dispersion in water with 10 % oleic acid in propilenglycol pretreatment) were
applied transdermally. The donor compartment contained 2 mL of 2 mg/mL losartan
potassium solution. The transport profiles were analyzed using two methods, i.e. lag
time diffusion method and compartmental modeling method.
The results showed that optimum conditions were gained at pH of 4,0 and
stirring rate of 350 rpm and the loading capacity was 47.7%. The mean of
nanoparticle size was 290.3 nm and the zeta potential was +50.79 mV. F IV had
higher rate of transport and could be applied transdermally in area of 48.58 cm2
to achieve therapeutic plasma level of 10 ng/mL. If the donor concentration of losartan
is increased to 10.79 mg/mL the application area is predicted to become 9 cm2.
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