| Summary: | We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa
layer with cumulus–oocyte complex is cultured in media supplemented with follicle stimulating hormone
(FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly
increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA,
expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not
detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and
CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting
that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44
antibody decreased the expression of procaspases suggesting that perturbation of HA–CD44 binding
leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA–CD44 binding is important
on apoptosis inhibitory mechanism in porcine granulosa cells
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