Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells
We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus–oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly...
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| Format: | Article |
| Language: | English |
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ELSEVIER
2009
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| Online Access: | https://repository.ugm.ac.id/91881/1/woro%20journal%201-Tunjung%20bbrc%202009.pdf |
| _version_ | 1797030987750703104 |
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| author | Tunjung, Woro Anindito Sri |
| author_facet | Tunjung, Woro Anindito Sri |
| author_sort | Tunjung, Woro Anindito Sri |
| collection | UGM |
| description | We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa
layer with cumulus–oocyte complex is cultured in media supplemented with follicle stimulating hormone
(FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly
increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA,
expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not
detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and
CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting
that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44
antibody decreased the expression of procaspases suggesting that perturbation of HA–CD44 binding
leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA–CD44 binding is important
on apoptosis inhibitory mechanism in porcine granulosa cells |
| first_indexed | 2024-03-13T22:14:40Z |
| format | Article |
| id | oai:generic.eprints.org:91881 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T22:14:40Z |
| publishDate | 2009 |
| publisher | ELSEVIER |
| record_format | dspace |
| spelling | oai:generic.eprints.org:918812014-09-23T03:42:15Z https://repository.ugm.ac.id/91881/ Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells Tunjung, Woro Anindito Sri Physiology We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus–oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA–CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA–CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells ELSEVIER 2009 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/91881/1/woro%20journal%201-Tunjung%20bbrc%202009.pdf Tunjung, Woro Anindito Sri (2009) Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells. Jurnal, 382. pp. 160-164. ISSN 0006-291X http://www.journals.elsevier.com/biochemical-and-biophysical-research-communications/ Biochemical and Biophysical Research Communications 382 (2009) |
| spellingShingle | Physiology Tunjung, Woro Anindito Sri Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title | Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title_full | Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title_fullStr | Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title_full_unstemmed | Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title_short | Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| title_sort | effect of hyaluronan to inhibit caspase activation in porcine granulosa cells |
| topic | Physiology |
| url | https://repository.ugm.ac.id/91881/1/woro%20journal%201-Tunjung%20bbrc%202009.pdf |
| work_keys_str_mv | AT tunjungworoaninditosri effectofhyaluronantoinhibitcaspaseactivationinporcinegranulosacells |