| Summary: | <p>There were many research on Phaleria macrocarpa (Scheff) boerl fruit for its activity as anticancer. However, there was none investigation on Phaleria macrocarpa (Scheff) boerl seed and fruit meat extract of ethanol effect against normal cell. The study conducted to identify the cytotoxicity of ethanol extract of Phaleria macrocarpa (Scheff) boerl seed dan fruit meat to mononuclear perifer normal cell of human blood and vero cell line. Besides, It was also investigated the p53 and Bcl-2 genes expression. The research consist of several sections including Phaleria macrocarpa (Scheff) boerl seed and fruit meat maceration with ethanol, cytotoxicity test to mononuclear perifer normal cell and vero cell line, and imunohistochemistry test to p53 and Bcl-2 genes expression. As positive control was used Tamoxifen dan 5-fluorourasil. The result showed that the ethanol extract of Phaleria macrocarpa (Scheff) boerl seed and fruit meat were not toxic to human mononuclear perifer normal cell, but slightly toxic to vero cell line. Tamoxifen and 5-fluorourasil were very toxic to mononuclear perifer normal cell and vero cell line. The ethanol extract of Phaleria macrocarpa (Scheff) boerl seed has higher cytotoxicity than fruit meat extract. The imunohistochemistry result explained that the ethanol extract of Phaleria macrocarpa (Scheff) boerl seed and fruit meat and tamoxifen did not increase p53 and decrease Bcl-2 genes expression. There was a possibility that the dying cell mechanism was caused by necrosis, not by apoptosis.</p>
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