PENGEMBANGAN IMMUNODOT BLOT DAN REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION UNTUK DIAGNOSA KOKSIDIOSIS Eimeria tenella
<p>Coccidiosis or bloody-diarrhea disease in chicken is the infectious diseases caused by one of the protozoan parasite Eimeria tenella. In Indonesia, the morbidity due to coccidiosis is about 80 to 90%. The rapid and accurate diagnoses needed to prevent the distribution of this disease. This...
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[Yogyakarta] : Fakultas Kedokteran Hewan UGM
2012
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| Summary: | <p>Coccidiosis or bloody-diarrhea disease in chicken is the infectious diseases caused by one of the protozoan parasite Eimeria tenella. In Indonesia, the morbidity due to coccidiosis is about 80 to 90%. The rapid and accurate diagnoses needed to prevent the distribution of this disease. This research aim is to develop the immunodt blot and reverse transcription-PCR methods for diagnosing of coccidiosis due to E. tenella infection. The oocysts sample of E. tenella were collected from the field and were propagated by injected the oocyst to the chickens, and collect the caecum after incubation periods. The oocysts, sporocysts, and sporozoites of E. tenella were then used for Western Blot and RT-PCR. The SDS-PAGE result showed that there were 5 protein bands found in the sporozoite of E. tenella with molecular weight between 15 to 91 kDa. These proteins were then tested with immunodot blot and gave positive reaction between antigen and antibody. The analysis was then continued with Western Blot but there were no antigenic protein profile found from this analysis. The RT-PCR result showed that the IHC-BiP gene was detected in sporocyst with molecular length between 700 &ndash |
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