| Summary: | <p>Background: Aggressive periodontitis is a disease of the tissues supporting teeth are characterized by rapid damage to the periodontal ligament and alveolar bone in young patients, generally occurs at age below 30 years. The process of tissue attachment loss and gingival recession four times faster than in chronic periodontitis . The main causes of aggressive periodontitis is Actinobacillus actinomycetemcomitans (A.actinomycetemcomitans). Several virulence factor are identified in this bacteria including adhesin, LPS, leucotoxin, cytoletal distending toxin (CDT), collagenase and outer membrane protein (OMP). This bacteria hasfimbriae that serve as adhesion and invasion, this fimbriae is a virulencefactors for infectionprocess in the oral cavity. A.actinomycetemcomitans also have nonfimbrial adhesin, that is Outer Membrane Protein (OMP). Purpose: The aim of this study was to determine and produce fimbrial and OMP adhesin A. actinomycetemcomitans with usability as a preventive therapy and to manage patients with aggressive periodontitis.. Methods: The early stages was to find specific fimbrial adhesin and OMP adhesin A.actinomycetemcomitans clinical isolate by SDS-PAGE analysis and hemagglutination test. Subgingival plaque were takenfrom patients with aggressiveperiodontitis.AAGMused for clinical isolated A. actinomycetemcomitans with added 75 ug/ml Bacitracin and 5 ug/ml Vancomycin.The omnimixer modification was used to cut A. actinomycetemcomitansfimbriae and OMPfor five and three times and every times was 15 minutes. Hemagglutination and SDS-PAGE test on fimbriae and OMP fractions considering that hemagglutinin protein can also function as an adhesin protein. Result: There were two hemagglutinin protein fimbriae and five major OMPs from A.actinomycetemcomitans. The molecular weight of hemaglutininprotein infimbrial adhesin were 53 kDa and 24 kDa andfor OMP adhesin i.e. 16 kDa, 28 kDa, 39 kDa, 66 kDa and 103 kDa. Conclusion: The 24 kDafimbrial adhesin, and 16 kDa, 28 kDa, 39 kDa, 66 kDa and 103 kDa OMP adhesin were an adhesin candidate from A. actinomycetemcomitans clinical isolate which is a material choice to manage patient with aggressive periodontitis.</p>
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