NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS

<p>Calcium hydroxide [Ca(OH2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OHh is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. Th...

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Main Author: , Maria Arifani
Format: Article
Published: [Yogyakarta] : Fak.Kedokteran Gigi Universitas Gadjah Mada 2012
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author , Maria Arifani
author_facet , Maria Arifani
author_sort , Maria Arifani
collection UGM
description <p>Calcium hydroxide [Ca(OH2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OHh is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The effect of Ca(OHh in NO regulation in pulp tissue cells is still unknown. As fibroblast is an important component in pulp tissue, the aim of this study was to investigate whether NO production is affected by Ca(OH)2 stimulation in fibroblast cells. Vero, a fibroblast-like cell line (2 x 104) was cultured in M199 culture medium. Overnight culture were stimulated with various concentrations of Ca(OH)2 solution (12,5 mg/ml, 25 mg/ml, and 50 mg/ml), or left untreated asnegative control, and then were incubated for 24 h at 37&deg
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institution Universiti Gadjah Mada
last_indexed 2024-03-13T22:28:45Z
publishDate 2012
publisher [Yogyakarta] : Fak.Kedokteran Gigi Universitas Gadjah Mada
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spelling oai:generic.eprints.org:961282014-11-28T07:34:33Z https://repository.ugm.ac.id/96128/ NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS , Maria Arifani <p>Calcium hydroxide [Ca(OH2] is a common antibacterial agent used in dental pulp therapy. It is known that Ca(OHh is able to induce cellular changes in pulp tissue. An inflammatory mediator nitric oxide (NO), functions in immune system and some cellular changes as in cell death mechanisms. The effect of Ca(OHh in NO regulation in pulp tissue cells is still unknown. As fibroblast is an important component in pulp tissue, the aim of this study was to investigate whether NO production is affected by Ca(OH)2 stimulation in fibroblast cells. Vero, a fibroblast-like cell line (2 x 104) was cultured in M199 culture medium. Overnight culture were stimulated with various concentrations of Ca(OH)2 solution (12,5 mg/ml, 25 mg/ml, and 50 mg/ml), or left untreated asnegative control, and then were incubated for 24 h at 37&deg [Yogyakarta] : Fak.Kedokteran Gigi Universitas Gadjah Mada 2012 Article NonPeerReviewed , Maria Arifani (2012) NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS. text. http://repository.ugm.ac.id/digitasi/index.php?module=cari_hasil_full&idbuku=3946
spellingShingle , Maria Arifani
NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title_full NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title_fullStr NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title_full_unstemmed NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title_short NITRIT OXIDE PRODUCTION RESPONSIVENESSTO THE EXPOSURE OF CALCIUM HYDROXIDE IN FIBROBLAST CELLS
title_sort nitrit oxide production responsivenessto the exposure of calcium hydroxide in fibroblast cells
work_keys_str_mv AT mariaarifani nitritoxideproductionresponsivenesstotheexposureofcalciumhydroxideinfibroblastcells