Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon
A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design s...
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| Format: | Article |
| Language: | English |
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I.J. Biotech.
2011
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| Online Access: | https://repository.ugm.ac.id/96929/1/Development%20of%20Random%20Amplified%20Polymorphism%20DNA%20Markers%20Linked.pdf |
| _version_ | 1826045074736152576 |
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| author | Daryono, Budi Setiadi Aristya, Ganies Riza Kasiamdari, Rina Sri |
| author_facet | Daryono, Budi Setiadi Aristya, Ganies Riza Kasiamdari, Rina Sri |
| author_sort | Daryono, Budi Setiadi |
| collection | UGM |
| description | A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. |
| first_indexed | 2024-03-13T22:31:33Z |
| format | Article |
| id | oai:generic.eprints.org:96929 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T22:31:33Z |
| publishDate | 2011 |
| publisher | I.J. Biotech. |
| record_format | dspace |
| spelling | oai:generic.eprints.org:969292014-12-05T07:04:17Z https://repository.ugm.ac.id/96929/ Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi Aristya, Ganies Riza Kasiamdari, Rina Sri Agricultural Land Management Agronomy Biological Sciences A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers. I.J. Biotech. 2011-12 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/96929/1/Development%20of%20Random%20Amplified%20Polymorphism%20DNA%20Markers%20Linked.pdf Daryono, Budi Setiadi and Aristya, Ganies Riza and Kasiamdari, Rina Sri (2011) Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon. Indonesian Journal of Biotechnology,, 16 (2). pp. 76-82. http://ijbiotech.ugm.ac.id/index.php/biotech/article/view/146 |
| spellingShingle | Agricultural Land Management Agronomy Biological Sciences Daryono, Budi Setiadi Aristya, Ganies Riza Kasiamdari, Rina Sri Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title | Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title_full | Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title_fullStr | Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title_full_unstemmed | Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title_short | Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon |
| title_sort | development of random amplified polymorphism dna markers linked to powdery mildew resistance gene in melon |
| topic | Agricultural Land Management Agronomy Biological Sciences |
| url | https://repository.ugm.ac.id/96929/1/Development%20of%20Random%20Amplified%20Polymorphism%20DNA%20Markers%20Linked.pdf |
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