| Summary: | Cucumber green mottle mosaic virus(CGMMV) and Kyuri green mottle mosaic virus(KGMMV) are seed borne
viruses, and they are also transmitted mechanically during agricultural practice and through water. Hence, these
viruses have potential diseases widely distributed throughout the world. Todetect different strains of CGMMV
and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The
results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in BaliIndonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia andCucumis metuliferus
isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using
two different ways of viral RNAs extraction. Based on the results, it could bereported that viral RNAs for cDNA
amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction
of each viruses infected leaves. In addition, results presented in this study demonstrated the application of
multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of
four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique
has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive
and reliable tool for detection of two major cucurbit infecting Tobamoviruses.
|
|---|