Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting Tobamovirus
Cucumber green mottle mosaic virus(CGMMV) and Kyuri green mottle mosaic virus(KGMMV) are seed borne viruses, and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. Todetect d...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Jordan Journal of Agricultural Sciences
2012
|
| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/96939/1/Application%20of%20Multiplex%20RT-PCR%20for%20Detection%20of%20Cucurbit-Infecting.pdf |
| _version_ | 1797031983947186176 |
|---|---|
| author | Daryono, Budi Setiadi Natsuaki, K.T. |
| author_facet | Daryono, Budi Setiadi Natsuaki, K.T. |
| author_sort | Daryono, Budi Setiadi |
| collection | UGM |
| description | Cucumber green mottle mosaic virus(CGMMV) and Kyuri green mottle mosaic virus(KGMMV) are seed borne
viruses, and they are also transmitted mechanically during agricultural practice and through water. Hence, these
viruses have potential diseases widely distributed throughout the world. Todetect different strains of CGMMV
and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The
results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in BaliIndonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia andCucumis metuliferus
isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using
two different ways of viral RNAs extraction. Based on the results, it could bereported that viral RNAs for cDNA
amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction
of each viruses infected leaves. In addition, results presented in this study demonstrated the application of
multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of
four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique
has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive
and reliable tool for detection of two major cucurbit infecting Tobamoviruses. |
| first_indexed | 2024-03-13T22:31:35Z |
| format | Article |
| id | oai:generic.eprints.org:96939 |
| institution | Universiti Gadjah Mada |
| language | English |
| last_indexed | 2024-03-13T22:31:35Z |
| publishDate | 2012 |
| publisher | Jordan Journal of Agricultural Sciences |
| record_format | dspace |
| spelling | oai:generic.eprints.org:969392014-12-05T07:04:55Z https://repository.ugm.ac.id/96939/ Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting Tobamovirus Daryono, Budi Setiadi Natsuaki, K.T. Agricultural Land Management Agronomy Cucumber green mottle mosaic virus(CGMMV) and Kyuri green mottle mosaic virus(KGMMV) are seed borne viruses, and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. Todetect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in BaliIndonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia andCucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could bereported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses. Jordan Journal of Agricultural Sciences 2012 Article PeerReviewed application/pdf en https://repository.ugm.ac.id/96939/1/Application%20of%20Multiplex%20RT-PCR%20for%20Detection%20of%20Cucurbit-Infecting.pdf Daryono, Budi Setiadi and Natsuaki, K.T. (2012) Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting Tobamovirus. Jordan Journal of Agricultural Sciences, 8 (1). pp. 46-55. ISSN 1815-8625 https://journals.ju.edu.jo/JJAS/article/viewFile/2931/2590 |
| spellingShingle | Agricultural Land Management Agronomy Daryono, Budi Setiadi Natsuaki, K.T. Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting Tobamovirus |
| title | Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting
Tobamovirus |
| title_full | Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting
Tobamovirus |
| title_fullStr | Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting
Tobamovirus |
| title_full_unstemmed | Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting
Tobamovirus |
| title_short | Application of Multiplex RT-PCR for Detection of Cucurbit-Infecting
Tobamovirus |
| title_sort | application of multiplex rt pcr for detection of cucurbit infecting tobamovirus |
| topic | Agricultural Land Management Agronomy |
| url | https://repository.ugm.ac.id/96939/1/Application%20of%20Multiplex%20RT-PCR%20for%20Detection%20of%20Cucurbit-Infecting.pdf |
| work_keys_str_mv | AT daryonobudisetiadi applicationofmultiplexrtpcrfordetectionofcucurbitinfectingtobamovirus AT natsuakikt applicationofmultiplexrtpcrfordetectionofcucurbitinfectingtobamovirus |