| Summary: | Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3
(CyHV-3), Koi herpes-like virus, and carp interstitial nephritis gill necrosis virus
(CNGV), caused significant morbidity (sickness or disease) and mortality in koi
and common carp (Cyprinus carpio). Mortality of KHV disease reached 80�100%
in affected populations. Glycoprotein has been used for vaccine development as
sub unit vaccine. The aim of this research was to clone and express membrane
glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV
gene was successfully cloned into pBSKS and sequenced. Result showed that
ORF124 KHV (isolate from Indonesian) had 100 % similarity with Cyprinid
herpesvirus 3 DNA strain TUMST1 (AP008984.1, isolate from Japan), 99%
similarity with Koi herpesvirus strain KHV-U (DQ657948.1, isolate from USA)
and Koi herpesvirus strain KHV-I (DQ177346.1, isolate from Israel). Prediction
analysis of T and B cell epitopes showed that ORF124 KHV protein can be used
as a vaccine candidate because had 14 and 11 T cell epitopes (IAd,
Rothbard/Taylor pattern), and had 10 B cell epitopes. ORF124 gene can be
expressed in pET32-a(+).
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