Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design
The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plac...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/9779/1/hafidz.pdf |
| _version_ | 1796875756565954560 |
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| author | Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin |
| author_facet | Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin |
| author_sort | Mahamad Maifiah, Mohd Hafidz |
| collection | IIUM |
| description | The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application. |
| first_indexed | 2024-03-05T22:43:04Z |
| format | Proceeding Paper |
| id | oai:generic.eprints.org:9779 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:43:04Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | oai:generic.eprints.org:97792021-06-23T06:49:44Z http://irep.iium.edu.my/9779/ Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin TA164 Bioengineering TP248.13 Biotechnology The gene encoding for phytase from ASUIA279 has been successfully cloned and expressed in pichia pastoris X-33 under the control of AOX1 promoter, named as HSUIA279(5H)AX. In this study, process and media components were screened for high yield phytase production in small scale culture by using plackett burman design. Three factors identified (Agitation, YNB, Yeast extract) were further optimized by Response Surface methodology (RSM) using Face Centered Composite Design (FCCD). Phytase activity in the optimized media (28 U/ml) increase 64% higher over than it basal media (17.004 U/ml) in the shaken flask culture. The cloning of the phytase enzyme from ASUIA279 into the Pichia pastoris system with minimal and potential media components would be a significant concern for a cost effective measure to produce a high yield and activity enzyme for animal feed application. 2011 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/9779/1/hafidz.pdf Mahamad Maifiah, Mohd Hafidz and Mohd. Salleh, Hamzah and Jamal, Parveen and Nuge, Tamrin (2011) Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TA164 Bioengineering TP248.13 Biotechnology Mahamad Maifiah, Mohd Hafidz Mohd. Salleh, Hamzah Jamal, Parveen Nuge, Tamrin Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title | Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title_full | Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title_fullStr | Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title_full_unstemmed | Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title_short | Screening and optimization of media components for phytase production of ASUIA 279 in Pichia pastoris system using statistical design |
| title_sort | screening and optimization of media components for phytase production of asuia 279 in pichia pastoris system using statistical design |
| topic | TA164 Bioengineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/9779/1/hafidz.pdf |
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