| Summary: | Cricula trifenestrata is one of the wild silkworms to be first grown in
Daerah Istimewa Yogyakarta (DIY) in 1995. C. trifenestrata is in the family of
Saturniidae with a special trait of its ability to produce golden cocoon. The wide
distribution of C. trifenestrata causes diversity. However, the study of genetic
diversity of C. trifenestrata has not been discovered yet.
This study is aimed to discover the genetic variation of C. trifenestrata
on five locations in DIY and two other locations in Central Java based on RAPD
molecular marker, to support and give clear classification. The morphological
characters were observed according to the color of wings, the body size, and the
wide spread of wings. Genetic variation was observed by using four RAPD
primers (OPA-01, OPA-02, OPA-11, OPA-20). The amplification result is
visualized through electrophoresis and the ribbon appeared was converted into
binary data to determine the percentage of polymorphic of C. trifenestrata. Cluster
analysis was done by using a method called Unweighted Pair-Group Methods
Using Arithmatic Averages (UPGMA) NTSys-PC.
The result of this study showed that the morphology of C. trifenestrata
are diverse. Genetic variation in each location is stated in polymorphic percentage,
i.e. Magelang (95,52%), Dlingo (93,75%), Playen (90,38%), Ngawen (88,89%),
Karang Tengah (83,82%), Wonogiri (83,02%) and Pakem (72,09%).
Amplification with four RAPD primers produce 101 polymorphic loccus and 1
monomorphic loccus with OPA-20 primer at 750 bp. C. trifenestrata from seven
locations of this research were classified into two main dendogram cluster at
similarity value of 0.65 (65%) and the majority of the species was clustered in one
group with a similarity of 0.67 (67%). C. trifenestrata from avocado with
percentage of polymorphic between 72-95%, from Spondias dulcis (kedondong)
with percentage of polymorphic between 90-93% and from cashew with
percentage of polymorphic between 83-88%.
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