| Summary: | This study was aimed to combine chicken legs skin gelatin and soy protein
isolate as a raw material for edible films. This research materials were used
chicken leg skin and soy protein isolate. The experimental was divided into two
stages. The first stage was processing of gelatin from chicken leg skin which
soaked in 0.1% NaOH, 0.1% sulfuric acid, and 0.4% citric acid. The second stage
was preparation of edible films. The material films were combination of soy
protein isolate and gelatin chicken legs skin in various ratio (75:25, 80:20, 85:15,
90:10, and 95:5). Statistical analysis were carried out by one Anova and the mean
difference was tested using Duncan's Multiple Range Test. SDS-PAGE result
showed the band pattern of the molecule was dominated by the protein molecules
of gelatin, SDS-PAGE pattern showed no changed of bands the type α1 and α2 of
edible films. The results of the analysis of amino acid content of edible films
using HPLC produced the largest amino acid residue composition in each
treatments is glysin ie 29.42%, 37.88%, 38.32%, 39.28% and 39.17% respetively.
This result described that protein profile of HPLC was dominated protein from
chicken leg gelatin. Observations with a scanning electron microscope have
showed the cross-linking between protein molecules of gelatin and soy protein
isolate. Different combinations of chicken legs skin gelatin and soy protein was
not different on the tensile strength, and elongation, but there were a significant
difference on the thickness and water vapor transmission rate. The fines quality of
edible films formed on 95:25.
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