PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS : PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS PADA AYAM BROILER
The experiments were conducted to determine some properties of the indigenous lactic acid bacteria (LAB) probiotics and its influence on biological expressions of broiler chickens. The LAB probiotics consisted of Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2) and Pediococcus acidilact...
| Main Authors: | , |
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| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2012
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| _version_ | 1797032167511949312 |
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| author | , Sri Harimurti, Ir.,SU. , Prof. Drs. Nasroedin, M.Sc |
| author_facet | , Sri Harimurti, Ir.,SU. , Prof. Drs. Nasroedin, M.Sc |
| author_sort | , Sri Harimurti, Ir.,SU. |
| collection | UGM |
| description | The experiments were conducted to determine some properties of the indigenous lactic
acid bacteria (LAB) probiotics and its influence on biological expressions of broiler chickens.
The LAB probiotics consisted of Lactobacillus murinus (Ar3), Streptococcus thermophilus
(Kp2) and Pediococcus acidilactici (Kd6) were assessed on their ability to adhere to the
chicken ileal epithelial cells (IEC) in vitro and in vivo as well. In the first stage of the
experiments were reared 30 broiler chickens as the collecting source of the IEC preparation to
the adhesion assay (in vitro) and 48 chickens were reared for sampling examination of SEM
(in vivo). In the second stage, the 60 broiler chickens were assigned randomly into 4 treatment
groups of LAB probiotics (mixed culture) orally supplementation consisted of (T0) one group
of unsupplemented and three groups of supplemented chickens as much as 107 (T1), 108 (T2)
and 109CFU/ml/bird/day (T3) respectively. All of treatment groups study the biological
expression consisted of productivity performance such as feed consumption, body weight
gain, feed conversion ratio and the intestinal histology structure such as villus height, villus
width and crypt depth (μm) in duodenum, jejunum and ileum. The third stage used 90
chickens for studying the competitive exclusion of Salmonella pullorum by LAB probiotic
strains. The broiler diet was formulated to meet the NRC recommendations and were
provided ad libitum. The data were analyzed using analysis of variance followed by Duncan
New Multiple Range Test. The results of the experiment in Part 1 showed that LAB probiotic
cells had a good ability to adhere to IEC as revealed by phase - contrast microscopy. The
scanning electron micrographs showed a clear appearance of Lactobacillus murinus (Ar3),
Streptococcus thermophilus (Kp2) and Pediococcus acidilactici (Kd6) attached firmly to the
intestine of the supplemented broiler but there were none on the intestine of unsupplemented
chicken. In Part 2, feed consumption, body weight gain and feed conversion ratio were
affected significantly (P<0.05) by the supplementation of LAB probiotics. Feed conversion
ratio were improved from 1.766 in unsupplemented group (T0) to 1.515 (T1), 1.496 (T2) and
1.457 (T3) respectively. Probiotics supplementation significantly increased villus height,
villus width, crypt depth in duodenum, jejunum and ileum which indicated the improvement
of the intestinal histology structure. The results of Part 3 showed that LAB probiotics
inhibited the growth of Salmonella pullorum in broiler intestine. The number of LAB count
(CFU/g) in the intestine of supplemented birds increased as much as 1.83 log cycle compared
to that of the control. In general, this experiment can be concluded that the indigenous LAB
probiotics fulfilled criterias of ideal broiler chicken probiotics. It had a good ability to adhere
to the intestine of broiler chicken, improved productivity performance, and had a good
property for protection against Salmonella pullorum. |
| first_indexed | 2024-03-13T22:34:20Z |
| format | Thesis |
| id | oai:generic.eprints.org:97931 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:34:20Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:979312016-03-04T08:49:00Z https://repository.ugm.ac.id/97931/ PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS : PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS PADA AYAM BROILER , Sri Harimurti, Ir.,SU. , Prof. Drs. Nasroedin, M.Sc ETD The experiments were conducted to determine some properties of the indigenous lactic acid bacteria (LAB) probiotics and its influence on biological expressions of broiler chickens. The LAB probiotics consisted of Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2) and Pediococcus acidilactici (Kd6) were assessed on their ability to adhere to the chicken ileal epithelial cells (IEC) in vitro and in vivo as well. In the first stage of the experiments were reared 30 broiler chickens as the collecting source of the IEC preparation to the adhesion assay (in vitro) and 48 chickens were reared for sampling examination of SEM (in vivo). In the second stage, the 60 broiler chickens were assigned randomly into 4 treatment groups of LAB probiotics (mixed culture) orally supplementation consisted of (T0) one group of unsupplemented and three groups of supplemented chickens as much as 107 (T1), 108 (T2) and 109CFU/ml/bird/day (T3) respectively. All of treatment groups study the biological expression consisted of productivity performance such as feed consumption, body weight gain, feed conversion ratio and the intestinal histology structure such as villus height, villus width and crypt depth (μm) in duodenum, jejunum and ileum. The third stage used 90 chickens for studying the competitive exclusion of Salmonella pullorum by LAB probiotic strains. The broiler diet was formulated to meet the NRC recommendations and were provided ad libitum. The data were analyzed using analysis of variance followed by Duncan New Multiple Range Test. The results of the experiment in Part 1 showed that LAB probiotic cells had a good ability to adhere to IEC as revealed by phase - contrast microscopy. The scanning electron micrographs showed a clear appearance of Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2) and Pediococcus acidilactici (Kd6) attached firmly to the intestine of the supplemented broiler but there were none on the intestine of unsupplemented chicken. In Part 2, feed consumption, body weight gain and feed conversion ratio were affected significantly (P<0.05) by the supplementation of LAB probiotics. Feed conversion ratio were improved from 1.766 in unsupplemented group (T0) to 1.515 (T1), 1.496 (T2) and 1.457 (T3) respectively. Probiotics supplementation significantly increased villus height, villus width, crypt depth in duodenum, jejunum and ileum which indicated the improvement of the intestinal histology structure. The results of Part 3 showed that LAB probiotics inhibited the growth of Salmonella pullorum in broiler intestine. The number of LAB count (CFU/g) in the intestine of supplemented birds increased as much as 1.83 log cycle compared to that of the control. In general, this experiment can be concluded that the indigenous LAB probiotics fulfilled criterias of ideal broiler chicken probiotics. It had a good ability to adhere to the intestine of broiler chicken, improved productivity performance, and had a good property for protection against Salmonella pullorum. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Sri Harimurti, Ir.,SU. and , Prof. Drs. Nasroedin, M.Sc (2012) PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS : PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS PADA AYAM BROILER. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=53360 |
| spellingShingle | ETD , Sri Harimurti, Ir.,SU. , Prof. Drs. Nasroedin, M.Sc PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS : PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS PADA AYAM BROILER |
| title | PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS :
PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS
PADA AYAM BROILER |
| title_full | PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS :
PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS
PADA AYAM BROILER |
| title_fullStr | PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS :
PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS
PADA AYAM BROILER |
| title_full_unstemmed | PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS :
PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS
PADA AYAM BROILER |
| title_short | PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS :
PENGARUHNYA TERHADAP EKSPRESI BIOLOGIS
PADA AYAM BROILER |
| title_sort | probiotik bakteri asam laktat indigenous pengaruhnya terhadap ekspresi biologis pada ayam broiler |
| topic | ETD |
| work_keys_str_mv | AT sriharimurtiirsu probiotikbakteriasamlaktatindigenouspengaruhnyaterhadapekspresibiologispadaayambroiler AT profdrsnasroedinmsc probiotikbakteriasamlaktatindigenouspengaruhnyaterhadapekspresibiologispadaayambroiler |