PERBANDINGAN MOTILITAS SPERMA POST-THAWING DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN ANTARA 4 MACAM METODE KRIOPRESERVASI YANG BERBEDA
Background: Frozen-thawed human spermatozoa are routinely used for many assisted reproduction programmes. However lower pregnancy rates have been reported using cryopreserved spermatozoa both intra uterine inseminations and IVF/ICSI with respect to the results obtained with fresh semen. These lower...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| _version_ | 1797032270223114240 |
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| author | , Hilwah Nora , Dr. Irwan Taufiqurrachman, SpOG(K) |
| author_facet | , Hilwah Nora , Dr. Irwan Taufiqurrachman, SpOG(K) |
| author_sort | , Hilwah Nora |
| collection | UGM |
| description | Background: Frozen-thawed human spermatozoa are routinely used for many
assisted reproduction programmes. However lower pregnancy rates have been
reported using cryopreserved spermatozoa both intra uterine inseminations and
IVF/ICSI with respect to the results obtained with fresh semen. These lower
pregnancy rates are attributed to a reduction of sperm motility induced by
cryopreservation procedures.
Objective: This experimental study compares the effect of four methods of
cryopreservation with and without addition of CPA, with respect to sperm motility
as the observed variable.
Research Design: Prospective Cohort
Methods: Thirty seven normozoospermic semen samples were taken for this
study through Sardjito infertility clinic, evaluated for its pre-freeze motility and
divided into two aliquots with and without CPA for each cryopreservation methods
then cryopreserved into 4 different methods. In Simple two steps freezing:
samples were cooled in 8°C and -4°C freezer, while in Simple graduated
freezing: cryostraws were placed in a freezer at -4°C. The Liquid nitrogen vapor
phase method cooling 1 cm above the surface of liquid nitrogen (-180°C) and
the last methods by directly submerged into liquid nitrogen. Thawing was done by
incubation at 37°C for 5 minutes. Thawed semen samples were evaluated for
their post-thaw motility.
Results: Sperm motility before cryopreservation was 52.9% ±4.5. The mean
motile sperm recovered for Direct plunge to liquid nitrogen freezing, Liquid
nitrogen vapor phase freezing, Simple graduated freezing and Simple two steps
freezing method with-CPA was 17%±7.83, 20.96%±5.81, 15.06%(SD±8.55) and
15.68%±8.3, respectively |
| first_indexed | 2024-03-13T22:36:02Z |
| format | Thesis |
| id | oai:generic.eprints.org:98435 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:36:02Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:984352016-03-04T08:47:01Z https://repository.ugm.ac.id/98435/ PERBANDINGAN MOTILITAS SPERMA POST-THAWING DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN ANTARA 4 MACAM METODE KRIOPRESERVASI YANG BERBEDA , Hilwah Nora , Dr. Irwan Taufiqurrachman, SpOG(K) ETD Background: Frozen-thawed human spermatozoa are routinely used for many assisted reproduction programmes. However lower pregnancy rates have been reported using cryopreserved spermatozoa both intra uterine inseminations and IVF/ICSI with respect to the results obtained with fresh semen. These lower pregnancy rates are attributed to a reduction of sperm motility induced by cryopreservation procedures. Objective: This experimental study compares the effect of four methods of cryopreservation with and without addition of CPA, with respect to sperm motility as the observed variable. Research Design: Prospective Cohort Methods: Thirty seven normozoospermic semen samples were taken for this study through Sardjito infertility clinic, evaluated for its pre-freeze motility and divided into two aliquots with and without CPA for each cryopreservation methods then cryopreserved into 4 different methods. In Simple two steps freezing: samples were cooled in 8°C and -4°C freezer, while in Simple graduated freezing: cryostraws were placed in a freezer at -4°C. The Liquid nitrogen vapor phase method cooling 1 cm above the surface of liquid nitrogen (-180°C) and the last methods by directly submerged into liquid nitrogen. Thawing was done by incubation at 37°C for 5 minutes. Thawed semen samples were evaluated for their post-thaw motility. Results: Sperm motility before cryopreservation was 52.9% ±4.5. The mean motile sperm recovered for Direct plunge to liquid nitrogen freezing, Liquid nitrogen vapor phase freezing, Simple graduated freezing and Simple two steps freezing method with-CPA was 17%±7.83, 20.96%±5.81, 15.06%(SD±8.55) and 15.68%±8.3, respectively [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Hilwah Nora and , Dr. Irwan Taufiqurrachman, SpOG(K) (2012) PERBANDINGAN MOTILITAS SPERMA POST-THAWING DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN ANTARA 4 MACAM METODE KRIOPRESERVASI YANG BERBEDA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=54801 |
| spellingShingle | ETD , Hilwah Nora , Dr. Irwan Taufiqurrachman, SpOG(K) PERBANDINGAN MOTILITAS SPERMA POST-THAWING DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN ANTARA 4 MACAM METODE KRIOPRESERVASI YANG BERBEDA |
| title | PERBANDINGAN MOTILITAS SPERMA POST-THAWING
DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN
ANTARA 4 MACAM METODE KRIOPRESERVASI YANG
BERBEDA |
| title_full | PERBANDINGAN MOTILITAS SPERMA POST-THAWING
DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN
ANTARA 4 MACAM METODE KRIOPRESERVASI YANG
BERBEDA |
| title_fullStr | PERBANDINGAN MOTILITAS SPERMA POST-THAWING
DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN
ANTARA 4 MACAM METODE KRIOPRESERVASI YANG
BERBEDA |
| title_full_unstemmed | PERBANDINGAN MOTILITAS SPERMA POST-THAWING
DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN
ANTARA 4 MACAM METODE KRIOPRESERVASI YANG
BERBEDA |
| title_short | PERBANDINGAN MOTILITAS SPERMA POST-THAWING
DENGAN ATAU TANPA MENGGUNAKAN KRIOPROTEKTAN
ANTARA 4 MACAM METODE KRIOPRESERVASI YANG
BERBEDA |
| title_sort | perbandingan motilitas sperma post thawing dengan atau tanpa menggunakan krioprotektan antara 4 macam metode kriopreservasi yang berbeda |
| topic | ETD |
| work_keys_str_mv | AT hilwahnora perbandinganmotilitasspermapostthawingdenganatautanpamenggunakankrioprotektanantara4macammetodekriopreservasiyangberbeda AT drirwantaufiqurrachmanspogk perbandinganmotilitasspermapostthawingdenganatautanpamenggunakankrioprotektanantara4macammetodekriopreservasiyangberbeda |