| Summary: | Fusarium species are fungal pathogen which produce mycotoxins, such as fumonisin,
trichothecene, and zearalenone. In this work, Fusarium species were isolated from
maize kernel in Tretep, identified morphologically, and confirmed by molecular
identification that used PCR assays and the partial sequence of TEF 1-α gene
(Translation Elongation Factor 1-α). The fumonisin-producing ability of Fusarium was
evaluated on corn medium and determined by ELISA (enzyme-linked immuno assay).
Among 9 isolates, three of them were identified as Fusarium verticillioides, two as
Fusarium temperatum, two as Fusarium globosum, one as Fusarium proliferatum, and
one as Fusarium subglutinans. F.temperatum is similar morphologically to
F.subglutinans, however both of their differences can be found by molecular analysis.
Fumonisin-producing abilities of Fusarium were determined in concentrations
20,51µg/g - 1109,74 µg/g medium.
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