Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung
Fusarium species are fungal pathogen which produce mycotoxins, such as fumonisin, trichothecene, and zearalenone. In this work, Fusarium species were isolated from maize kernel in Tretep, identified morphologically, and confirmed by molecular identification that used PCR assays and the partial seque...
| المؤلفون الرئيسيون: | , |
|---|---|
| التنسيق: | أطروحة |
| منشور في: |
[Yogyakarta] : Universitas Gadjah Mada
2012
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| الموضوعات: |
| _version_ | 1826045419457609728 |
|---|---|
| author | , Petrus Adityo Nugroho , Prof.Dr.Ir. Endang S.Rahayu, MS., |
| author_facet | , Petrus Adityo Nugroho , Prof.Dr.Ir. Endang S.Rahayu, MS., |
| author_sort | , Petrus Adityo Nugroho |
| collection | UGM |
| description | Fusarium species are fungal pathogen which produce mycotoxins, such as fumonisin,
trichothecene, and zearalenone. In this work, Fusarium species were isolated from
maize kernel in Tretep, identified morphologically, and confirmed by molecular
identification that used PCR assays and the partial sequence of TEF 1-α gene
(Translation Elongation Factor 1-α). The fumonisin-producing ability of Fusarium was
evaluated on corn medium and determined by ELISA (enzyme-linked immuno assay).
Among 9 isolates, three of them were identified as Fusarium verticillioides, two as
Fusarium temperatum, two as Fusarium globosum, one as Fusarium proliferatum, and
one as Fusarium subglutinans. F.temperatum is similar morphologically to
F.subglutinans, however both of their differences can be found by molecular analysis.
Fumonisin-producing abilities of Fusarium were determined in concentrations
20,51µg/g - 1109,74 µg/g medium. |
| first_indexed | 2024-03-13T22:36:50Z |
| format | Thesis |
| id | oai:generic.eprints.org:98702 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:36:50Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:987022016-03-04T08:48:15Z https://repository.ugm.ac.id/98702/ Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung , Petrus Adityo Nugroho , Prof.Dr.Ir. Endang S.Rahayu, MS., ETD Fusarium species are fungal pathogen which produce mycotoxins, such as fumonisin, trichothecene, and zearalenone. In this work, Fusarium species were isolated from maize kernel in Tretep, identified morphologically, and confirmed by molecular identification that used PCR assays and the partial sequence of TEF 1-α gene (Translation Elongation Factor 1-α). The fumonisin-producing ability of Fusarium was evaluated on corn medium and determined by ELISA (enzyme-linked immuno assay). Among 9 isolates, three of them were identified as Fusarium verticillioides, two as Fusarium temperatum, two as Fusarium globosum, one as Fusarium proliferatum, and one as Fusarium subglutinans. F.temperatum is similar morphologically to F.subglutinans, however both of their differences can be found by molecular analysis. Fumonisin-producing abilities of Fusarium were determined in concentrations 20,51µg/g - 1109,74 µg/g medium. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Petrus Adityo Nugroho and , Prof.Dr.Ir. Endang S.Rahayu, MS., (2012) Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55357 |
| spellingShingle | ETD , Petrus Adityo Nugroho , Prof.Dr.Ir. Endang S.Rahayu, MS., Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title | Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title_full | Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title_fullStr | Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title_full_unstemmed | Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title_short | Identifikasi Molekuler Fusarium Penghasil Fumonisin yang Diisolasi dari Biji Jagung (Zea mays) di Tretep, Temanggung |
| title_sort | identifikasi molekuler fusarium penghasil fumonisin yang diisolasi dari biji jagung zea mays di tretep temanggung |
| topic | ETD |
| work_keys_str_mv | AT petrusadityonugroho identifikasimolekulerfusariumpenghasilfumonisinyangdiisolasidaribijijagungzeamaysditreteptemanggung AT profdrirendangsrahayums identifikasimolekulerfusariumpenghasilfumonisinyangdiisolasidaribijijagungzeamaysditreteptemanggung |