Identifikasi Black Aspergilli Penghasil Okratoksin A pada Komoditas Pertanian di Yogyakarta dengan Metode Molekuler
Agriculture products are susceptible contaminated with ochratoxin A (OTA), commonly produced by several fungi such as Penicillium verrucosum, Penicillium nordicum, Aspergillus section nigri or black aspergilli especially A.carbonarius, dan A.niger. This research was focused on black aspergilli isola...
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| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2012
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| _version_ | 1797032411823865856 |
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| author | , APRIYANTO DWI NUGROHO , Prof. Dr. Ir. Endang S. Rahayu, MS., |
| author_facet | , APRIYANTO DWI NUGROHO , Prof. Dr. Ir. Endang S. Rahayu, MS., |
| author_sort | , APRIYANTO DWI NUGROHO |
| collection | UGM |
| description | Agriculture products are susceptible contaminated with ochratoxin A (OTA),
commonly produced by several fungi such as Penicillium verrucosum, Penicillium
nordicum, Aspergillus section nigri or black aspergilli especially A.carbonarius,
dan A.niger. This research was focused on black aspergilli isolated from
fermented cocoa, coffee beans and dried cassava in Yogyakarta, and designed to
identify species within black aspergilli group and their OTA ability. Identification
black aspergilli at species level will be useful to make clear relationship between
OTA contamination on food product and the toxin producer. Identification of the
isolate were carried out by using macroscopic as well as microscopic characters
on identification media and confirmed by specific PCR assays. Four isolates
(YAC-9, YAK-6, YAK-12, YAG-3) were found as OTA producing-strain, with
the highest OTA found on YES solid medium was 57,68 ppb. Based on
morphological characters, 16 isolates can be grouped into 5 species, after
confirmed by molecular data, the groups was identified as A.carbonarius, A.
niger, A. tubingensis, A aculeatus, dan A.ibericus. OTA producing-strains were
identified as A.carbonarius and A. niger, meanwhile A. tubingensis, A aculeatus,
dan A.ibericus was found non OTA producing-strains. |
| first_indexed | 2024-03-13T22:38:01Z |
| format | Thesis |
| id | oai:generic.eprints.org:99066 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:38:01Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:990662016-03-04T08:45:20Z https://repository.ugm.ac.id/99066/ Identifikasi Black Aspergilli Penghasil Okratoksin A pada Komoditas Pertanian di Yogyakarta dengan Metode Molekuler , APRIYANTO DWI NUGROHO , Prof. Dr. Ir. Endang S. Rahayu, MS., ETD Agriculture products are susceptible contaminated with ochratoxin A (OTA), commonly produced by several fungi such as Penicillium verrucosum, Penicillium nordicum, Aspergillus section nigri or black aspergilli especially A.carbonarius, dan A.niger. This research was focused on black aspergilli isolated from fermented cocoa, coffee beans and dried cassava in Yogyakarta, and designed to identify species within black aspergilli group and their OTA ability. Identification black aspergilli at species level will be useful to make clear relationship between OTA contamination on food product and the toxin producer. Identification of the isolate were carried out by using macroscopic as well as microscopic characters on identification media and confirmed by specific PCR assays. Four isolates (YAC-9, YAK-6, YAK-12, YAG-3) were found as OTA producing-strain, with the highest OTA found on YES solid medium was 57,68 ppb. Based on morphological characters, 16 isolates can be grouped into 5 species, after confirmed by molecular data, the groups was identified as A.carbonarius, A. niger, A. tubingensis, A aculeatus, dan A.ibericus. OTA producing-strains were identified as A.carbonarius and A. niger, meanwhile A. tubingensis, A aculeatus, dan A.ibericus was found non OTA producing-strains. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , APRIYANTO DWI NUGROHO and , Prof. Dr. Ir. Endang S. Rahayu, MS., (2012) Identifikasi Black Aspergilli Penghasil Okratoksin A pada Komoditas Pertanian di Yogyakarta dengan Metode Molekuler. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55401 |
| spellingShingle | ETD , APRIYANTO DWI NUGROHO , Prof. Dr. Ir. Endang S. Rahayu, MS., Identifikasi Black Aspergilli Penghasil Okratoksin A pada Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title | Identifikasi Black Aspergilli Penghasil Okratoksin A pada
Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title_full | Identifikasi Black Aspergilli Penghasil Okratoksin A pada
Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title_fullStr | Identifikasi Black Aspergilli Penghasil Okratoksin A pada
Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title_full_unstemmed | Identifikasi Black Aspergilli Penghasil Okratoksin A pada
Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title_short | Identifikasi Black Aspergilli Penghasil Okratoksin A pada
Komoditas Pertanian di Yogyakarta dengan Metode Molekuler |
| title_sort | identifikasi black aspergilli penghasil okratoksin a pada komoditas pertanian di yogyakarta dengan metode molekuler |
| topic | ETD |
| work_keys_str_mv | AT apriyantodwinugroho identifikasiblackaspergillipenghasilokratoksinapadakomoditaspertaniandiyogyakartadenganmetodemolekuler AT profdrirendangsrahayums identifikasiblackaspergillipenghasilokratoksinapadakomoditaspertaniandiyogyakartadenganmetodemolekuler |