STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD
Physical defect in human bones can be healed with bone grafting process. Most of the materials used in the process of bone grafting are the autograft. Autograft has some strengths and weaknesses. Some of the weaknesses are the increasing of the infection risk and blood loss, the increasing anesthesi...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| _version_ | 1797032493360087040 |
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| author | , Eko Pujiyanto, S.Si.,MT. , Ir. Alva Edy Tontowi, MSc., Ph.D. |
| author_facet | , Eko Pujiyanto, S.Si.,MT. , Ir. Alva Edy Tontowi, MSc., Ph.D. |
| author_sort | , Eko Pujiyanto, S.Si.,MT. |
| collection | UGM |
| description | Physical defect in human bones can be healed with bone grafting process. Most of the
materials used in the process of bone grafting are the autograft. Autograft has some
strengths and weaknesses. Some of the weaknesses are the increasing of the infection risk
and blood loss, the increasing anesthesia time and the limited amount of it. These
weaknesses support the opportunity to develop synthetic bone graft. One of the synthetic
bone graft function is scaffold. Hydroxyapatite (HA) is one of the material that mostly
used for synthetic bone graft as scaffold. One of the material which can be used to produce
HA is gypsum. Indonesia has a great potential for local gypsum with low prices. Local
gypsum can be used as material base for produce synthetic bone graft as scaffold
(synthetic scaffold).
The purpose of this study was to synthesis of the local gypsum into HAg powder. Find out
the chemical, physical and cytotoxicity properties of HAg powder. Find out the
characterization of powder deposition and sintering process to produce synthetic scaffold.
Find out chemical properties, porosity, compressive strength and cytotoxicity properties
synthetic scaffold.
Wet reaction method assisted by microwave is used to react of local gypsum and
diammonium hydrogen phosphate into HAg. Synthetic scaffold was made by powder
deposition and sintering from TCPg-PMMA mixing powder. HAg, HAk and synthetic
scaffold characterizations including physical and chemical properties were conducted by
AAS, XRD, XRF, mercury porosimetry and SEM-EDX. In vitro cytotoxicity testing of
HAg, HAk and synthetic scaffold were conducted by MTT method using vero cells.
Characterization results of HAg powder are rounded, agglomeration and the purity up to
100%. XRD pattern of HAg is similar to HAk and closed to JCPDS No.09-432 (HA).
EDX test showed molar ratio Ca/P of HAg is 1.77 closed to theoretical is 1.67 that HAg
have a lot of Ca. In vitro cytotoxicity test showed that there was no significant difference
in cytotoxicity properties of HAg and HAk. Calcination of HAg at temperature 800oC with
holding time 2 hours has changed phase HA to tricalcium phosphate (TCPg). Synthetic
scaffold has porosity on the interval 62.79% to 69.67% and compressive strength on the
interval 1.53 MPa to 3.71 MPa. The best synthetic scaffold has porosity is 62.79% with
compressive strength is 3.706 MPa. Mercury porosimetry test showed, the best synthetic
scaffold has inter-connective porosity up to 88.25%, pore size is at 0.05-355 μm with
median pore is 52.64 μm. Sintering process at 1450oC with heating rate 8oC/min and
holding time 2 hours has changed phase HA to TCP but does not effect on in vitro
cytotoxicity properties of on synthetic scaffold.
The conclusion of this study was local gypsum Tasikmalaya has been successfully
synthesized into HAg. The best synthetic scaffold can be made with powder deposition
process and sintering. The sintering process effects on the chemical-physical properties,
porosity and compressive strength, but it does not effect on cytotoxicity properties of
synthetic scaffold. The best synthetic scaffold could potentially be applied in the medical
field with some further research. |
| first_indexed | 2024-03-13T22:39:14Z |
| format | Thesis |
| id | oai:generic.eprints.org:99465 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:39:14Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:994652016-03-04T08:46:28Z https://repository.ugm.ac.id/99465/ STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD , Eko Pujiyanto, S.Si.,MT. , Ir. Alva Edy Tontowi, MSc., Ph.D. ETD Physical defect in human bones can be healed with bone grafting process. Most of the materials used in the process of bone grafting are the autograft. Autograft has some strengths and weaknesses. Some of the weaknesses are the increasing of the infection risk and blood loss, the increasing anesthesia time and the limited amount of it. These weaknesses support the opportunity to develop synthetic bone graft. One of the synthetic bone graft function is scaffold. Hydroxyapatite (HA) is one of the material that mostly used for synthetic bone graft as scaffold. One of the material which can be used to produce HA is gypsum. Indonesia has a great potential for local gypsum with low prices. Local gypsum can be used as material base for produce synthetic bone graft as scaffold (synthetic scaffold). The purpose of this study was to synthesis of the local gypsum into HAg powder. Find out the chemical, physical and cytotoxicity properties of HAg powder. Find out the characterization of powder deposition and sintering process to produce synthetic scaffold. Find out chemical properties, porosity, compressive strength and cytotoxicity properties synthetic scaffold. Wet reaction method assisted by microwave is used to react of local gypsum and diammonium hydrogen phosphate into HAg. Synthetic scaffold was made by powder deposition and sintering from TCPg-PMMA mixing powder. HAg, HAk and synthetic scaffold characterizations including physical and chemical properties were conducted by AAS, XRD, XRF, mercury porosimetry and SEM-EDX. In vitro cytotoxicity testing of HAg, HAk and synthetic scaffold were conducted by MTT method using vero cells. Characterization results of HAg powder are rounded, agglomeration and the purity up to 100%. XRD pattern of HAg is similar to HAk and closed to JCPDS No.09-432 (HA). EDX test showed molar ratio Ca/P of HAg is 1.77 closed to theoretical is 1.67 that HAg have a lot of Ca. In vitro cytotoxicity test showed that there was no significant difference in cytotoxicity properties of HAg and HAk. Calcination of HAg at temperature 800oC with holding time 2 hours has changed phase HA to tricalcium phosphate (TCPg). Synthetic scaffold has porosity on the interval 62.79% to 69.67% and compressive strength on the interval 1.53 MPa to 3.71 MPa. The best synthetic scaffold has porosity is 62.79% with compressive strength is 3.706 MPa. Mercury porosimetry test showed, the best synthetic scaffold has inter-connective porosity up to 88.25%, pore size is at 0.05-355 μm with median pore is 52.64 μm. Sintering process at 1450oC with heating rate 8oC/min and holding time 2 hours has changed phase HA to TCP but does not effect on in vitro cytotoxicity properties of on synthetic scaffold. The conclusion of this study was local gypsum Tasikmalaya has been successfully synthesized into HAg. The best synthetic scaffold can be made with powder deposition process and sintering. The sintering process effects on the chemical-physical properties, porosity and compressive strength, but it does not effect on cytotoxicity properties of synthetic scaffold. The best synthetic scaffold could potentially be applied in the medical field with some further research. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Eko Pujiyanto, S.Si.,MT. and , Ir. Alva Edy Tontowi, MSc., Ph.D. (2012) STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=55974 |
| spellingShingle | ETD , Eko Pujiyanto, S.Si.,MT. , Ir. Alva Edy Tontowi, MSc., Ph.D. STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title | STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title_full | STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title_fullStr | STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title_full_unstemmed | STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title_short | STUDI PEMANFAATAN GIPSUM LOKAL SEBAGAI BAHAN DASAR BONE GRAFT SINTESIS YANG BERFUNGSI SEBAGAI SCAFFOLD |
| title_sort | studi pemanfaatan gipsum lokal sebagai bahan dasar bone graft sintesis yang berfungsi sebagai scaffold |
| topic | ETD |
| work_keys_str_mv | AT ekopujiyantossimt studipemanfaatangipsumlokalsebagaibahandasarbonegraftsintesisyangberfungsisebagaiscaffold AT iralvaedytontowimscphd studipemanfaatangipsumlokalsebagaibahandasarbonegraftsintesisyangberfungsisebagaiscaffold |