| Summary: | Immunostimulant is one of the preventive mechanism against infectious diseases. Traditional medicine has been used by our ancestors to maintain their health. One ingredient of traditional medicines widely used is sirih merah leaves (Piper crocatum Pav. & Ruiz). However, there is no scientific evidence yet and responsible compounds for this activity are studied or published. Therefore, this study was aimed to isolate and identify responsible active compounds present in sirih merah leaves (P. crocatum), and possibly determine their immunostimulant potency. Preliminary results showed that ethanolic extracts of sirih merah leaves displayed non-specific immunomodulatory effects on rat's immune response [50 ug/ml (116 latex), 25 ug/ml (115 latex), 5 ug/ml (99 latex) per 100 macrophages]. This ethanolic extract was subjected into partition, fractionation directed by bioassay. Active compounds present in the active fraction obtained were separated, purified by preparative thin layer chromatography to give the two compounds 9compounds 1 and 20. Two active compounds were tested for their purity by TLC using three different mobile phases and compounds identification was done by spectrometric methode such as UV, IR, GC-MS and NMR. Based on spectra data obtained, compound 1 was identified as 2-allyl-4- (1'-hydroxy-1'(3" ,4" ,5"-trimethoxyphenyl) propan-2'-yl)-3,5-dimethoxycyclohexa 3,5-dienone dan isolat 2 adalah 2-ally-4-(1'-acetyl-1'-(3" ,4" ,5"-trimethoxyphenyl) propan-2'-yl)-3,5-dimethoxycyclohexa-3,5-dienone. The immunostimulant activity compound 1 and 2 (5 ug/ml each) were comparable to positive control and tested at equal doses.
|
|---|