SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR)
Fe3O4 nanoparticles have been successfully fabricated by co-precipitation method. To study the potency of Fe3O4 as active material on surface plasmon resonance based biosensor application, the magnetic properties, modifying the surface of Fe3O4 nanoparticles by making a single layer system using pol...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2012
|
| Subjects: |
| _version_ | 1826045646478508032 |
|---|---|
| author | , Agus Riyanto , Dr. Edi Suharyadi |
| author_facet | , Agus Riyanto , Dr. Edi Suharyadi |
| author_sort | , Agus Riyanto |
| collection | UGM |
| description | Fe3O4 nanoparticles have been successfully fabricated by co-precipitation
method. To study the potency of Fe3O4 as active material on surface plasmon
resonance based biosensor application, the magnetic properties, modifying the
surface of Fe3O4 nanoparticles by making a single layer system using
polyethylene glycol 4000 (PEG-4000), and binding of the modified nanoparticles
to biomolecules (α-amylase) have been also investigated. Fe3O4 nanoparticles was
synthesized from 1 mol FeSO4.7H2O and 2 mol FeCl3.6H2O by co-precipitation
method by controlling the synthesis temperature, concentration of co-precipitan
(NH4OH), and duration of centrifugation. The results of x-ray diffraction (XRD)
and transmission electron microscopy (TEM) analysis showed that Fe3O4
nanoparticles with the smallest grain size and low levels of impurities can be
obtained by increasing the concentration of co-precipitan, and holding of
temperature synthesis at room temperature (about 30
o
C), and to extend the
duration of centrifugation. The result of measurement using vibrating sample
magnetometer (VSM) showed that the smaller the grain size (diameter) Fe3O4
nanoparticles, the higher the magnetic response in the nanoparticles. In addition,
the smaller the grain diameter of nanoparticles Fe3O4 nanoparticles coercivity also
decreases. Analysis of infra-red spectroscopy (IR spectroscopy) showed that the
surface of Fe3O4 nanoparticles can be modified with PEG-4000 through a covalent
bond Fe-O. Modification of Fe3O4 nanoparticles was proven to be increase
dispersibility nanoparticles in a liquid. In addition, the results of IR spectroscopy
analysis showed that PEG-4000 layer can bind to the target biomolecules (α-
amylase). Thus, in particular Fe3O4 nanoparticles with very small grain size has a
great potency can be harnessed to improve the immobilization of biomolecules on
the sensing surface of SPR based biosensor. |
| first_indexed | 2024-03-13T22:40:18Z |
| format | Thesis |
| id | oai:generic.eprints.org:99817 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:40:18Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:998172016-03-04T08:45:30Z https://repository.ugm.ac.id/99817/ SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) , Agus Riyanto , Dr. Edi Suharyadi ETD Fe3O4 nanoparticles have been successfully fabricated by co-precipitation method. To study the potency of Fe3O4 as active material on surface plasmon resonance based biosensor application, the magnetic properties, modifying the surface of Fe3O4 nanoparticles by making a single layer system using polyethylene glycol 4000 (PEG-4000), and binding of the modified nanoparticles to biomolecules (α-amylase) have been also investigated. Fe3O4 nanoparticles was synthesized from 1 mol FeSO4.7H2O and 2 mol FeCl3.6H2O by co-precipitation method by controlling the synthesis temperature, concentration of co-precipitan (NH4OH), and duration of centrifugation. The results of x-ray diffraction (XRD) and transmission electron microscopy (TEM) analysis showed that Fe3O4 nanoparticles with the smallest grain size and low levels of impurities can be obtained by increasing the concentration of co-precipitan, and holding of temperature synthesis at room temperature (about 30 o C), and to extend the duration of centrifugation. The result of measurement using vibrating sample magnetometer (VSM) showed that the smaller the grain size (diameter) Fe3O4 nanoparticles, the higher the magnetic response in the nanoparticles. In addition, the smaller the grain diameter of nanoparticles Fe3O4 nanoparticles coercivity also decreases. Analysis of infra-red spectroscopy (IR spectroscopy) showed that the surface of Fe3O4 nanoparticles can be modified with PEG-4000 through a covalent bond Fe-O. Modification of Fe3O4 nanoparticles was proven to be increase dispersibility nanoparticles in a liquid. In addition, the results of IR spectroscopy analysis showed that PEG-4000 layer can bind to the target biomolecules (α- amylase). Thus, in particular Fe3O4 nanoparticles with very small grain size has a great potency can be harnessed to improve the immobilization of biomolecules on the sensing surface of SPR based biosensor. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Agus Riyanto and , Dr. Edi Suharyadi (2012) SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56173 |
| spellingShingle | ETD , Agus Riyanto , Dr. Edi Suharyadi SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title | SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title_full | SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title_fullStr | SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title_full_unstemmed | SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title_short | SINTESIS NANOPARTIKEL Fe3O4 (MAGNETIT) DAN POTENSINYA SEBAGAI MATERIAL AKTIF PADA PERMUKAAN SENSING BIOSENSOR BERBASIS SURFACE PLASMON RESONANCE (SPR) |
| title_sort | sintesis nanopartikel fe3o4 magnetit dan potensinya sebagai material aktif pada permukaan sensing biosensor berbasis surface plasmon resonance spr |
| topic | ETD |
| work_keys_str_mv | AT agusriyanto sintesisnanopartikelfe3o4magnetitdanpotensinyasebagaimaterialaktifpadapermukaansensingbiosensorberbasissurfaceplasmonresonancespr AT dredisuharyadi sintesisnanopartikelfe3o4magnetitdanpotensinyasebagaimaterialaktifpadapermukaansensingbiosensorberbasissurfaceplasmonresonancespr |