AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT SERTA IDENTIFIKASI SENYAWA AKTIFNYA
Citrus fruit peel is a major waste in citrus fruit processing industry. Peel of some citrus fruit has been used empirically to treat acne. Ethyl acetate extract of fresh citrus fruit peel is known to have a greater antibacterial activity than essential oil. Therefore, studies of antibacterial activi...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| author | , Rima Munawaroh, S.Si., Apt. , Prof. Dr. Suwidjiyo Pramono, DEA., Apt. |
| author_facet | , Rima Munawaroh, S.Si., Apt. , Prof. Dr. Suwidjiyo Pramono, DEA., Apt. |
| author_sort | , Rima Munawaroh, S.Si., Apt. |
| collection | UGM |
| description | Citrus fruit peel is a major waste in citrus fruit processing industry. Peel
of some citrus fruit has been used empirically to treat acne. Ethyl acetate extract
of fresh citrus fruit peel is known to have a greater antibacterial activity than
essential oil. Therefore, studies of antibacterial activity of ethyl acetate extract of
Citrus spp. fruit peel and ethyl acetate fraction rest of the most active extract
distillation against acne-inducing bacteria and identification of active compound
was done.
Antibacterial activity of ethyl acetate extract of Citrus aurantifolia
(Christm. & Panz.) Swingle (lime), Citrus hystrix DC. (kaffir lime), and Citrus
nobilis Lour. (tangerines) fresh fruit peel were tested against Propionibacterium
acnes ATCC 6919, Staphylococcus aureus ATCC 25923, and Staphylococcus
epidermidis by pour plate diffusion method. The most active extract (kaffir lime
rind) was distilled by water distillation to separate essential oil fraction, then
distillation residue was partitioned with ethyl acetate to obtain ethyl acetate and
water fractions. MIC (minimum inhibitory concentration) and MBC (minimum
bactericidal concentration) values of the most active fraction (ethyl acetate
fraction) were determined by broth dilution method. Spot of ethyl acetate fraction
that has antibacterial proved by bioautography test was isolated using
preparative TLC (thin layer chromatography). Structure of active isolate was
identified based on UV, IR, 1H NMR, 13C NMR, 1H-13C HMQC, 1H-1H COSY, 1H-
13C HMBC, MS spectra and compared to data from literature.
MIC and MBC values of ethyl acetate fraction against S. epidermidis were
1.13 mg/ml and 2.25 mg/ml, respectively, while MIC and MBC values of ethyl
acetate fraction against S. aureus were 0.56 mg/ml and 1.13 mg/ml, respectively.
Chemical structure of active isolate was identified as furanocoumarin
oxypeucedanin hydrate or 5-(2,3-dihydroxy-3-methylbutoxy) psoralen. |
| first_indexed | 2024-03-13T22:40:25Z |
| format | Thesis |
| id | oai:generic.eprints.org:99852 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:40:25Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:998522016-03-04T08:48:34Z https://repository.ugm.ac.id/99852/ AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT SERTA IDENTIFIKASI SENYAWA AKTIFNYA , Rima Munawaroh, S.Si., Apt. , Prof. Dr. Suwidjiyo Pramono, DEA., Apt. ETD Citrus fruit peel is a major waste in citrus fruit processing industry. Peel of some citrus fruit has been used empirically to treat acne. Ethyl acetate extract of fresh citrus fruit peel is known to have a greater antibacterial activity than essential oil. Therefore, studies of antibacterial activity of ethyl acetate extract of Citrus spp. fruit peel and ethyl acetate fraction rest of the most active extract distillation against acne-inducing bacteria and identification of active compound was done. Antibacterial activity of ethyl acetate extract of Citrus aurantifolia (Christm. & Panz.) Swingle (lime), Citrus hystrix DC. (kaffir lime), and Citrus nobilis Lour. (tangerines) fresh fruit peel were tested against Propionibacterium acnes ATCC 6919, Staphylococcus aureus ATCC 25923, and Staphylococcus epidermidis by pour plate diffusion method. The most active extract (kaffir lime rind) was distilled by water distillation to separate essential oil fraction, then distillation residue was partitioned with ethyl acetate to obtain ethyl acetate and water fractions. MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values of the most active fraction (ethyl acetate fraction) were determined by broth dilution method. Spot of ethyl acetate fraction that has antibacterial proved by bioautography test was isolated using preparative TLC (thin layer chromatography). Structure of active isolate was identified based on UV, IR, 1H NMR, 13C NMR, 1H-13C HMQC, 1H-1H COSY, 1H- 13C HMBC, MS spectra and compared to data from literature. MIC and MBC values of ethyl acetate fraction against S. epidermidis were 1.13 mg/ml and 2.25 mg/ml, respectively, while MIC and MBC values of ethyl acetate fraction against S. aureus were 0.56 mg/ml and 1.13 mg/ml, respectively. Chemical structure of active isolate was identified as furanocoumarin oxypeucedanin hydrate or 5-(2,3-dihydroxy-3-methylbutoxy) psoralen. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Rima Munawaroh, S.Si., Apt. and , Prof. Dr. Suwidjiyo Pramono, DEA., Apt. (2012) AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT SERTA IDENTIFIKASI SENYAWA AKTIFNYA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56085 |
| spellingShingle | ETD , Rima Munawaroh, S.Si., Apt. , Prof. Dr. Suwidjiyo Pramono, DEA., Apt. AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title | AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH
JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK
KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI
EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT
SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title_full | AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH
JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK
KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI
EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT
SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title_fullStr | AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH
JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK
KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI
EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT
SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title_full_unstemmed | AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH
JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK
KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI
EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT
SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title_short | AKTIVITAS ANTIBAKTERI EKSTRAK ETIL ASETAT KULIT BUAH
JERUK NIPIS (Citrus aurantifolia), JERUK PURUT (Citrus hystrix), JERUK
KEPROK (Citrus nobilis), DAN FRAKSI ETIL ASETAT SISA DESTILASI
EKSTRAK TERAKTIF TERHADAP BAKTERI PENYEBAB JERAWAT
SERTA IDENTIFIKASI SENYAWA AKTIFNYA |
| title_sort | aktivitas antibakteri ekstrak etil asetat kulit buah jeruk nipis citrus aurantifolia jeruk purut citrus hystrix jeruk keprok citrus nobilis dan fraksi etil asetat sisa destilasi ekstrak teraktif terhadap bakteri penyebab jerawat serta identifikasi senyawa aktifnya |
| topic | ETD |
| work_keys_str_mv | AT rimamunawarohssiapt aktivitasantibakteriekstraketilasetatkulitbuahjeruknipiscitrusaurantifoliajerukpurutcitrushystrixjerukkeprokcitrusnobilisdanfraksietilasetatsisadestilasiekstrakteraktifterhadapbakteripenyebabjerawatsertaidentifikasisenyawaaktifnya AT profdrsuwidjiyopramonodeaapt aktivitasantibakteriekstraketilasetatkulitbuahjeruknipiscitrusaurantifoliajerukpurutcitrushystrixjerukkeprokcitrusnobilisdanfraksietilasetatsisadestilasiekstrakteraktifterhadapbakteripenyebabjerawatsertaidentifikasisenyawaaktifnya |