POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO
Signal transduction disregulation, i.e. MAP kinase pathway leads to degenerative disease development, including cancer. Today, people are seeking for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness and decrease its side effect. Curcumin is a potential chemopreventive a...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| author | , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., |
| author_facet | , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., |
| author_sort | , Endah Puspitasari |
| collection | UGM |
| description | Signal transduction disregulation, i.e. MAP kinase pathway leads to
degenerative disease development, including cancer. Today, people are seeking
for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness
and decrease its side effect. Curcumin is a potential chemopreventive agent
targeting kinase proteins on their ATP binding site. Thus, Faculty of Pharmacy
UGM developed curcumin analogue, PGV-0. Due to its insolubility, Na PGV-0
was then synthesized. This research was done to study Na PGV-0 potential as cochemotherapeutic
agent targeting proteins involved in MAP kinase pathway,
especially EGFR and IKK, and its effector protein, Pgp and COX-2.
Co-chemotherapeutic activity was determined by cytotoxicity single and in
combination with doxorubicin on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr
cells using MTT assay. While the molecular mechanisms were explored in silico
using PLANTS software. Na PGV-0 as ligand was prepared using Marvin Sketch,
while protein targets were prepared using YASARA.
Na PGV-0 exhibited cytotoxic property on MCF-7 ori, MCF-7/DOX,
HeLa, and WiDr cells with IC50 values of 52.33 + 6.36 |
| first_indexed | 2024-03-13T22:40:25Z |
| format | Thesis |
| id | oai:generic.eprints.org:99855 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:40:25Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:998552016-03-04T08:46:32Z https://repository.ugm.ac.id/99855/ POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., ETD Signal transduction disregulation, i.e. MAP kinase pathway leads to degenerative disease development, including cancer. Today, people are seeking for co-chemotherapeutic agent to increase chemotherapeutic agent�s effectiveness and decrease its side effect. Curcumin is a potential chemopreventive agent targeting kinase proteins on their ATP binding site. Thus, Faculty of Pharmacy UGM developed curcumin analogue, PGV-0. Due to its insolubility, Na PGV-0 was then synthesized. This research was done to study Na PGV-0 potential as cochemotherapeutic agent targeting proteins involved in MAP kinase pathway, especially EGFR and IKK, and its effector protein, Pgp and COX-2. Co-chemotherapeutic activity was determined by cytotoxicity single and in combination with doxorubicin on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr cells using MTT assay. While the molecular mechanisms were explored in silico using PLANTS software. Na PGV-0 as ligand was prepared using Marvin Sketch, while protein targets were prepared using YASARA. Na PGV-0 exhibited cytotoxic property on MCF-7 ori, MCF-7/DOX, HeLa, and WiDr cells with IC50 values of 52.33 + 6.36 [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Endah Puspitasari and , Dr. Agung Endro Nugroho, M.Si., Apt., (2012) POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56219 |
| spellingShingle | ETD , Endah Puspitasari , Dr. Agung Endro Nugroho, M.Si., Apt., POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN TERHADAP BERBAGAI SEL KANKER DAN PENELUSURAN TARGET MOLEKULERNYA PADA JALUR MAP KINASE: PENDEKATAN IN VITRO DAN IN SILICO |
| title | POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_full | POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_fullStr | POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_full_unstemmed | POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_short | POTENSI KO-KEMOTERAPI Na PGV-0 DENGAN DOXORUBICIN
TERHADAP BERBAGAI SEL KANKER
DAN PENELUSURAN TARGET MOLEKULERNYA
PADA JALUR MAP KINASE:
PENDEKATAN IN VITRO DAN IN SILICO |
| title_sort | potensi ko kemoterapi na pgv 0 dengan doxorubicin terhadap berbagai sel kanker dan penelusuran target molekulernya pada jalur map kinase pendekatan in vitro dan in silico |
| topic | ETD |
| work_keys_str_mv | AT endahpuspitasari potensikokemoterapinapgv0dengandoxorubicinterhadapberbagaiselkankerdanpenelusurantargetmolekulernyapadajalurmapkinasependekataninvitrodaninsilico AT dragungendronugrohomsiapt potensikokemoterapinapgv0dengandoxorubicinterhadapberbagaiselkankerdanpenelusurantargetmolekulernyapadajalurmapkinasependekataninvitrodaninsilico |