Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi Leucocephala Tannin dan Penggantian Plasma Seminal Terhadap Viabilitas dan Fertilitas Spermatozoa
This research is a true designed experiment, aimed to develop a new (novel) method in preparation of fresh sperms of goat that able to maintain the functional viability and fertility of spermatozoa at storage temperature of 14 � 15°C for 14 days, and can be able to applied for AI effectively and...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| author | , Mirajuddin , Ir. Kustono, M.Sc., Ph.D. |
| author_facet | , Mirajuddin , Ir. Kustono, M.Sc., Ph.D. |
| author_sort | , Mirajuddin |
| collection | UGM |
| description | This research is a true designed experiment, aimed to develop a new (novel) method in
preparation of fresh sperms of goat that able to maintain the functional viability and
fertility of spermatozoa at storage temperature of 14 � 15°C for 14 days, and can be
able to applied for AI effectively and efficientlly. The study is targeted as a solution for
the failure of the implementation of AI in the area that is the presence of frozen sperm
and liquid nitrogen are limited. The research was carried out in three stages, in which
the fresh sperm preparation method through supplementation of various doses of
Leucocepahala tannin (LT) per ml of semen with extender glucose 18.9%, as phase I,
the replacement of seminal plasma with Earle'S medium supplement (Earle�S balanced
salt solution: EBSS) and 100 μl hypotaurine-2mM l(-1) EBSS, as phase II, and the AI
applications using fresh sperm from experiment I and II, as phase III. The results
showed that supplementation of LT2,5% per ml of semen in the extender 18.9% glucose
and seminal plasma replacement with EBSS medium, a fresh sperm of goat preparation
method is best in terms of recovery on the percentage of sperm viability and normal
morphology on the storage. Both method improve pregnancy rate (conception rate:
CR) resulteds from the first AI service of 95.38% and 100%, and the kidding rate (KR)
are 75.38% and 72.72%, respectively. |
| first_indexed | 2024-03-13T22:40:34Z |
| format | Thesis |
| id | oai:generic.eprints.org:99901 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:40:34Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:999012016-03-04T08:47:48Z https://repository.ugm.ac.id/99901/ Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi Leucocephala Tannin dan Penggantian Plasma Seminal Terhadap Viabilitas dan Fertilitas Spermatozoa , Mirajuddin , Ir. Kustono, M.Sc., Ph.D. ETD This research is a true designed experiment, aimed to develop a new (novel) method in preparation of fresh sperms of goat that able to maintain the functional viability and fertility of spermatozoa at storage temperature of 14 � 15°C for 14 days, and can be able to applied for AI effectively and efficientlly. The study is targeted as a solution for the failure of the implementation of AI in the area that is the presence of frozen sperm and liquid nitrogen are limited. The research was carried out in three stages, in which the fresh sperm preparation method through supplementation of various doses of Leucocepahala tannin (LT) per ml of semen with extender glucose 18.9%, as phase I, the replacement of seminal plasma with Earle'S medium supplement (Earle�S balanced salt solution: EBSS) and 100 μl hypotaurine-2mM l(-1) EBSS, as phase II, and the AI applications using fresh sperm from experiment I and II, as phase III. The results showed that supplementation of LT2,5% per ml of semen in the extender 18.9% glucose and seminal plasma replacement with EBSS medium, a fresh sperm of goat preparation method is best in terms of recovery on the percentage of sperm viability and normal morphology on the storage. Both method improve pregnancy rate (conception rate: CR) resulteds from the first AI service of 95.38% and 100%, and the kidding rate (KR) are 75.38% and 72.72%, respectively. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Mirajuddin and , Ir. Kustono, M.Sc., Ph.D. (2012) Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi Leucocephala Tannin dan Penggantian Plasma Seminal Terhadap Viabilitas dan Fertilitas Spermatozoa. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56060 |
| spellingShingle | ETD , Mirajuddin , Ir. Kustono, M.Sc., Ph.D. Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi Leucocephala Tannin dan Penggantian Plasma Seminal Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title | Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi
Leucocephala Tannin dan Penggantian Plasma Seminal
Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title_full | Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi
Leucocephala Tannin dan Penggantian Plasma Seminal
Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title_fullStr | Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi
Leucocephala Tannin dan Penggantian Plasma Seminal
Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title_full_unstemmed | Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi
Leucocephala Tannin dan Penggantian Plasma Seminal
Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title_short | Rekayasa Preparasi Sperma Cair Kambing melalui Suplementasi
Leucocephala Tannin dan Penggantian Plasma Seminal
Terhadap Viabilitas dan Fertilitas Spermatozoa |
| title_sort | rekayasa preparasi sperma cair kambing melalui suplementasi leucocephala tannin dan penggantian plasma seminal terhadap viabilitas dan fertilitas spermatozoa |
| topic | ETD |
| work_keys_str_mv | AT mirajuddin rekayasapreparasispermacairkambingmelaluisuplementasileucocephalatannindanpenggantianplasmaseminalterhadapviabilitasdanfertilitasspermatozoa AT irkustonomscphd rekayasapreparasispermacairkambingmelaluisuplementasileucocephalatannindanpenggantianplasmaseminalterhadapviabilitasdanfertilitasspermatozoa |