Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe
Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distributio...
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| Format: | Article |
| Language: | English |
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Research Management Institute (RMI)
2013
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| Online Access: | https://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf |
| _version_ | 1796899826187632640 |
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| author | Ismail, Normah Mohamad Kharoe, Nur' Ain |
| author_facet | Ismail, Normah Mohamad Kharoe, Nur' Ain |
| author_sort | Ismail, Normah |
| collection | UITM |
| description | Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed
for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity ofboth the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated
that the proteases protein bands fall between 10 to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in
proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could
be a successful method to partially purify proteases from bilimbi especially from the unripe stage. |
| first_indexed | 2024-03-06T01:26:39Z |
| format | Article |
| id | oai:ir.uitm.edu.my:13046 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T01:26:39Z |
| publishDate | 2013 |
| publisher | Research Management Institute (RMI) |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:130462016-05-30T04:30:54Z https://ir.uitm.edu.my/id/eprint/13046/ Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe srj Ismail, Normah Mohamad Kharoe, Nur' Ain Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity ofboth the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between 10 to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. Research Management Institute (RMI) 2013 Article PeerReviewed text en https://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe. (2013) Scientific Research Journal <https://ir.uitm.edu.my/view/publication/Scientific_Research_Journal/>, 10 (2). pp. 29-49. ISSN 1675-7009 https://srj.uitm.edu.my/ |
| spellingShingle | Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Ismail, Normah Mohamad Kharoe, Nur' Ain Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title | Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title_full | Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title_fullStr | Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title_full_unstemmed | Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title_short | Extraction and partial purification of protease from Bilimbi (Averrhoa bilimbi L.) / Normah Ismail and Nur' Ain Mohamad Kharoe |
| title_sort | extraction and partial purification of protease from bilimbi averrhoa bilimbi l normah ismail and nur ain mohamad kharoe |
| topic | Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate |
| url | https://ir.uitm.edu.my/id/eprint/13046/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2013.pdf |
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