Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni
Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutr...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF |
| _version_ | 1825734560181125120 |
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| author | Rosni, Nordalilah |
| author_facet | Rosni, Nordalilah |
| author_sort | Rosni, Nordalilah |
| collection | UITM |
| description | Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption. |
| first_indexed | 2024-03-06T01:33:42Z |
| format | Thesis |
| id | oai:ir.uitm.edu.my:15907 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T01:33:42Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:159072022-03-10T02:00:01Z https://ir.uitm.edu.my/id/eprint/15907/ Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni Rosni, Nordalilah Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Palm Puree (PP) is a value-added product prepared from squeezed fruitlets of oil palm fresh fruit bunches. Fiber was incorporated at different percentages for samples T24 and PT99 at 2% and 5% fiber incorporated for each sample, respectively. The study was carried out to determine the potential nutritional benefits of PP such as neuroprotection, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) inhibition and antimicrobial properties. Neuroprotection properties were observed in infant brain cancer; human neuroblastoma cell line SH-SY5Y differentiated by treatment of retinoic acid and induced with H2O2. The samples gave negative neurotoxicity results and promising neuroprotective effects on 80% confluent SHSY5Y cell line in 96 well plates at a dose dependent manner. PT99 displayed most protection against H2 0 2 -induced cell lines and followed by T24 at 100 (ig/mL (p<0.05). The HMGR inhibition assay aims to find the cholesterol lowering ability of PP using pravastatin, a known HMGR inhibitor as control. T24/5 gave a considerably better oxidation of NADPH at 0.159 Units/mgP, followed by PT99/5 (0.009 Units/mgP) as compared to pravastatin. Antimicrobial testing against selected pathogenic bacteria and fungi infecting human were performed using well diffusion method at concentrations of 200 (ig/ml, 300 (ig/ml, 500 (xg/ml, 800 fig/ml and 1 mg/ml. The test showed negative results as no inhibitory activities were observed. The HPLC analysis showed that the four vitamin E derivatives identified in all PP samples were a-T3, y-T3, 8-T3 and a-TP. T24 exhibited significantly (p<0.05) higher total amount of vitamin E as compared to PT99. In conclusion, PP can be used as a healthy value-added product for human consumption. 2015 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni. (2015) Masters thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/15907.pdf> |
| spellingShingle | Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Rosni, Nordalilah Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title | Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title_full | Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title_fullStr | Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title_full_unstemmed | Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title_short | Neuroprotection, HMGR inhibition, vitamin E quantification and antimicrobial properties of palm puree / Nordalilah Rosni |
| title_sort | neuroprotection hmgr inhibition vitamin e quantification and antimicrobial properties of palm puree nordalilah rosni |
| topic | Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) |
| url | https://ir.uitm.edu.my/id/eprint/15907/1/TM_NORDALILAH%20ROSNI%20AS%2015_5.PDF |
| work_keys_str_mv | AT rosninordalilah neuroprotectionhmgrinhibitionvitaminequantificationandantimicrobialpropertiesofpalmpureenordalilahrosni |