Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian
In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), et...
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| Format: | Thesis |
| Language: | English |
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2013
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| Online Access: | https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf |
| _version_ | 1825735010553954304 |
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| author | Sufian, Adila Sahida |
| author_facet | Sufian, Adila Sahida |
| author_sort | Sufian, Adila Sahida |
| collection | UITM |
| description | In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7). |
| first_indexed | 2024-03-06T01:40:36Z |
| format | Thesis |
| id | oai:ir.uitm.edu.my:18282 |
| institution | Universiti Teknologi MARA |
| language | English |
| last_indexed | 2024-03-06T01:40:36Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oai:ir.uitm.edu.my:182822022-03-01T02:05:42Z https://ir.uitm.edu.my/id/eprint/18282/ Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian Sufian, Adila Sahida In the present work, isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura (Elaeocarpacea) was carried out based on bioassay-guided approach. The methanol leaf extract of M. calabura (MCME) and its partitioned extracts [petroleum ether (MCPE), ethyl acetate (MCEA) and aqueous (MCAQ)] were subjected to antimicrobial and cytotoxic activities using micro-broth dilution and MTT assays, respectively. MCEA, which appeared to be the most active extract against MSS A (S. aureus 25923) (MIC - 125 μg/mL) and HL60 cell line (human acute promyelocytic leukemia) (IC50 = 17.26 μg/mL), was further fractionated using vacuum liquid chromatography (VLC) to afford seven fractions (F1-F7). 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian. (2013) Masters thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/18282.pdf> |
| spellingShingle | Sufian, Adila Sahida Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title | Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title_full | Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title_fullStr | Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title_full_unstemmed | Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title_short | Bioassay-guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of Muntingia calabura / Adila Sahida Sufian |
| title_sort | bioassay guided isolation and identification of antimicrobial and cytotoxic compounds from the leaves of muntingia calabura adila sahida sufian |
| url | https://ir.uitm.edu.my/id/eprint/18282/2/TM_ADILA%20SAHIDA%20SUFIAN%20%20PH%2013_5.pdf |
| work_keys_str_mv | AT sufianadilasahida bioassayguidedisolationandidentificationofantimicrobialandcytotoxiccompoundsfromtheleavesofmuntingiacalaburaadilasahidasufian |