Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak

A research has being conducted on Archidendron pauciflorum obtained from argo market at Kuala Pilah. The study aim is to determine the presence of antioxidant properties of Archidendron pauciflorum by using DPPH assay, which potentially can be used in pharmaceutical and human health. The samples wer...

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Main Author: Ishak, Mohd Khairi
Format: Student Project
Language:English
Published: 2017
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/33522/1/33522.pdf
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author Ishak, Mohd Khairi
author_facet Ishak, Mohd Khairi
author_sort Ishak, Mohd Khairi
collection UITM
description A research has being conducted on Archidendron pauciflorum obtained from argo market at Kuala Pilah. The study aim is to determine the presence of antioxidant properties of Archidendron pauciflorum by using DPPH assay, which potentially can be used in pharmaceutical and human health. The samples were extracted by using sonicator. The explant such as seeds and pods were screening for antioxidant activity. The highest percentage of scavenging activity was showed by seeds (91.87%) followed by pods (85%). Then, these two samples were further tested to investigate the toxicity level of Archidendron pauciflorum by using brine shrimp lethality assay and also determination of lethal concentration LC50 as a standard toxicity indicator. Explant that showed highest lethal concentration LC50 effected in 24 hours was seeds (1.944 mg/ml) followed by pods (1.884 mg/ml). This two sample showed low toxicity level since the lethal concentration was higher than 1 mg/ml. This study showed that explant might become potential sources since as it has high antioxidant with low toxicity level. Then, these two were continues study to determine the proximate analysis. Explant that showed the higher carbohydrate and protein was seeds (84.45%) and (3.33%) while pods (76.84%) and (2.03%). But seeds were lower in moisture, ash and fat content (8.85%), (3.17%) and (0.20%) compared to pod (10.67%), (10.23%) and (0.23%). This study showed that explant also might become the potential to produce healthy snack since it has high carbohydrate and protein content.
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spelling oai:ir.uitm.edu.my:335222020-08-12T08:36:03Z https://ir.uitm.edu.my/id/eprint/33522/ Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak Ishak, Mohd Khairi Laboratories. General works Extraction (Chemistry) Antioxidants A research has being conducted on Archidendron pauciflorum obtained from argo market at Kuala Pilah. The study aim is to determine the presence of antioxidant properties of Archidendron pauciflorum by using DPPH assay, which potentially can be used in pharmaceutical and human health. The samples were extracted by using sonicator. The explant such as seeds and pods were screening for antioxidant activity. The highest percentage of scavenging activity was showed by seeds (91.87%) followed by pods (85%). Then, these two samples were further tested to investigate the toxicity level of Archidendron pauciflorum by using brine shrimp lethality assay and also determination of lethal concentration LC50 as a standard toxicity indicator. Explant that showed highest lethal concentration LC50 effected in 24 hours was seeds (1.944 mg/ml) followed by pods (1.884 mg/ml). This two sample showed low toxicity level since the lethal concentration was higher than 1 mg/ml. This study showed that explant might become potential sources since as it has high antioxidant with low toxicity level. Then, these two were continues study to determine the proximate analysis. Explant that showed the higher carbohydrate and protein was seeds (84.45%) and (3.33%) while pods (76.84%) and (2.03%). But seeds were lower in moisture, ash and fat content (8.85%), (3.17%) and (0.20%) compared to pod (10.67%), (10.23%) and (0.23%). This study showed that explant also might become the potential to produce healthy snack since it has high carbohydrate and protein content. 2017 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/33522/1/33522.pdf Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak. (2017) [Student Project] (Unpublished)
spellingShingle Laboratories. General works
Extraction (Chemistry)
Antioxidants
Ishak, Mohd Khairi
Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title_full Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title_fullStr Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title_full_unstemmed Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title_short Antioxidant, toxicity and proximate analysis of archidendron pauciflorum (Jering) / Mohd Khairi Ishak
title_sort antioxidant toxicity and proximate analysis of archidendron pauciflorum jering mohd khairi ishak
topic Laboratories. General works
Extraction (Chemistry)
Antioxidants
url https://ir.uitm.edu.my/id/eprint/33522/1/33522.pdf
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