Detection of escherichia coli from chili paste at retail market / Aisyah Ahmad

Red chili is an important food ingredient in Malaysia and easily obtained at the market. The aims of the study were to determine the presence as well as the antibiotic resistance profile of Escherichia coli inside a chili paste commonly sold at the hypermarket and wet market. From the combination of...

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Bibliographic Details
Main Author: Ahmad, Aisyah
Format: Student Project
Language:English
Published: 2018
Subjects:
Online Access:https://ir.uitm.edu.my/id/eprint/33649/1/33649.pdf
Description
Summary:Red chili is an important food ingredient in Malaysia and easily obtained at the market. The aims of the study were to determine the presence as well as the antibiotic resistance profile of Escherichia coli inside a chili paste commonly sold at the hypermarket and wet market. From the combination of serial dilution up to the concentration of 10-3, Escherichia coli were detected only in Sample A out of six samples tested with a density ranging between 7.3x10-2 to 3.12x10-3 CFU/g respectively. A total of six isolates results were obtained in this study by IMViC identification methods has indicate that Escherichia coli was not a high health risk to the consumer. However, public should be aware of the need for improving hygienic standards among the food handlers. Proliferation of antibiotic and multi antibiotic resistant bacteria is a public health treat worldwide. All the six Escherichia coli isolates were tested toward five antibiotics and found to be resistant towards Cephalothin (KF30) and Penicillin (P10) tested and susceptible towards Imipenem (IPM10), Norfloxacin (NOR10) and Tetracycline (TE30) with two antibiotic resistance patterns. The MAR index values of 0.4 indicate that the isolates were exposed to high risk sources in the environment. Therefore, public should be aware and always practice proper food handling.